Photoactivatable aggregation-induced emission probes for lipid droplets-specific live cell imaging

被引：138

作者：

Gao, Meng ^{[1
]}

Su, Huifang ^{[2
]}

Lin, Yuhan ^{[1
]}

Ling, Xia ^{[1
]}

Li, Shiwu ^{[1
]}

Qin, Anjun ^{[1
]}

Tang, Ben Zhong ^{[1
,2
]}

机构：

[1] South China Univ Technol, State Key Lab Luminescent Mat & Devices, Guangdong Innovat Res Team, Guangzhou 510640, Guangdong, Peoples R China

[2] Hong Kong Univ Sci & Technol, Chinese Natl Engn Res Ctr Tissue Restorat & Recon, Dept Chem, Hong Kong Branch, Kowloon, Hong Kong, Peoples R China

来源：

CHEMICAL SCIENCE | 2017年 / 8卷 / 03期

基金：

美国国家科学基金会; 中国博士后科学基金;

关键词：

FLUORESCENCE; MICROSCOPY; FLUOROPHORES; DYNAMICS; BIOPROBE; TOOLS; RESTRICTION; MECHANISM; ORGANELLE; POLARITY;

D O I：

10.1039/c6sc04842k

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Photoactivatable probes for lipid droplets (LDs)-specific live-cell imaging are powerful tools for investigating their biological functions through precise spatial and temporal control. Ideal photoactivatable probes for LDs imaging require high concentration accumulation of fluorophores in LDs, simple synthetic procedures, and excellent photoactivation efficiency. However, it is difficult to overcome these challenges by conventional fluorophores due to aggregation-caused quenching (ACQ). In this study, a new class of photoactivatable and LDs- specific fluorescent probes was developed based on dihydro-2-azafluorenones, which can easily undergo photooxidative dehydrogenation reaction to afford 2-azafluorenones with aggregation-induced emission (AIE) properties. Dihydro-2-azafluorenones as photoactivatable and LDs-specific probes display significant advantages of excellent photoactivation efficiency and lack of self-quenching in the aggregated state, and are expected to have broad applications in study of biological functions of LDs' through light-controlled spatiotemporal imaging.

引用

页码：1763 / 1768

页数：6

共 53 条

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