Coexpression of CdSe and CdSe/CdS quantum dots in live cells using molecular hyperspectral imaging technology

被引：8

作者：

Li, Qingli ^{[1
]}

Peng, Hui ^{[1
]}

Wang, Jing ^{[2
]}

Wang, Yiting ^{[2
]}

Guo, Fangmin ^{[1
]}

机构：

[1] E China Normal Univ, Shanghai Key Lab Multidimens Informat Proc, Shanghai 200241, Peoples R China

[2] E China Normal Univ, Inst Adv Interdisciplinary Res, Shanghai 200062, Peoples R China

来源：

JOURNAL OF BIOMEDICAL OPTICS | 2015年 / 20卷 / 11期

基金：

中国国家自然科学基金;

关键词：

spectral imaging; quantum dots; image detection systems; medical and biological imaging;

D O I：

10.1117/1.JBO.20.11.110504

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A direct spatial and spectral observation of CdSe and CdSe/CdS quantum dots (QDs) as probes in live cells is performed by using a custom molecular hyperspectral imaging (MHI) system. Water-soluble CdSe and CdSe/CdS QDs are synthesized in aqueous solution under the assistance of high-intensity ultrasonic irradiation and incubated with colon cancer cells for bioimaging. Unlike the traditional fluorescence microscopy methods, MHI technology can identify QD probes according to their spectral signatures and generate coexpression and stain titer maps by a clustering method. The experimental results show that the MHI method has potential to unmix biomarkers by their spectral information, which opens up a pathway of optical multiplexing with many different QD probes. (C) 2015 Society of Photo-Optical Instrumentation Engineers (SPIE)

引用

页数：4

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