MicroRNA-223 triggers inflammation in porcine aorta by activating NLRP3 inflammasome under selenium deficiency

被引:19
作者
Zhang, Qing [1 ]
Zhang, Kaixin [1 ]
Han, Yanfei [1 ]
Zhang, Yiming [1 ]
Xue, Hua [2 ]
Zhou, Ling [2 ]
Shi, Guangliang [1 ]
Li, Shu [1 ]
机构
[1] Northeast Agr Univ, Dept Basic Vet Med, Coll Vet Med, Harbin, Peoples R China
[2] Natl Selenium Rich Prod Qual Supervis & Inspect C, Enshi, Peoples R China
关键词
microRNA‐ 223; NLRP3; porcine; selenium deficient; vascular injury; ENDOTHELIAL-CELLS; DIETARY SELENIUM; VITAMIN-E; APOPTOSIS; EXPRESSION; DYSFUNCTION; DISEASE;
D O I
10.1002/jcp.30178
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Selenium (Se) is an essential trace element in organism. Se deficiency can cause many diseases, including vascular disease. Studies have shown that inflammation is the main inducement of vascular disease, microRNA (miRNA) can influence inflammation in various ways, and Se deficiency can affect miRNAs expression. To study the mechanism of aorta damage caused by Se deficiency, we constructed a Se deficiency porcine aorta model and found that Se deficiency can significantly inhibit miR-223, which downregulates the expression of nucleotide-binding oligomerization domain-like receptor family 3 (NLRP3). Subsequently, we found that in Se deficiency group, NLRP3, and its downstream (caspase-1, apoptosis-related spot-like protein [ASC], IL-18, IL-1 beta) expression was significantly increased. In vitro, we cultured pig iliac endothelium cell lines, and constructed miR-223 knockdown and overexpression models. NLRP3 messenger RNA and protein levels were significant increased in the knockdown group, and decreased in the overexpression group. The results of this study show that Se deficiency in porcine arteries can induce inflammation through miR-223/NLRP3.
引用
收藏
页码:4555 / 4564
页数:10
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