Infusion of epidermal growth factor and basic fibroblast growth factor into the striatum of parkinsonian rats leads to in vitro proliferation and differentiation of adult neural progenitor cells

This study investigated the proliferation and differentiation of adult neural progenitor cells (aNPCs) derived from the striatum and substantia nigra (SN) of parkinsonian rats. We found that aNPCs isolated from the two areas of parkinsonian rats readily formed nestin-enriched neurospheres in vitro and exhibited an ability to differentiate into either neurons or astrocytes. Injection of epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF) into the striatum of parkinsonian rats prior to the harvesting striatal aNPCs significantly increased the neurosphere formation rate and multiple differentiation capacity of these aNPCs when cultured in vitro. These data suggest that striatal and nigral adult NPCs in parkinsonian rats retain the abilities of proliferation and differentiation in vitro. In addition, exogenously applied growth factors could up-regulate the developmental potential of aNPCs. We conclude that our data supports the notion that endogenous cell replacement therapies may be useful for the future treatment of Parkinson's disease (PD). (C) 2004 Elsevier Ireland Ltd. All rights reserved.