Optimizing Medium Components to Enhance High Cell Mass Production of Biotherapeutic Strain Lactobacillus reuteri DSM 20016T by Statistical Method

被引：0

作者：

Selvamani, S. ^{[1
,2
]}

Dailin, D. J. ^{[1
,2
]}

Rostom, M. ^{[3
]}

Malek, R. A. ^{[1
]}

Gupta, V. K. ^{[4
]}

El-Enshasy, H. A. ^{[1
,2
,5
]}

机构：

[1] Univ Teknol Malaysia, Inst Bioprod Dev, Skudai 81310, Johor, Malaysia

[2] Univ Teknol Malaysia, Fac Engn, Sch Chem & Energy Engn, Dept Bioproc & Polymer Engn, Skudai, Malaysia

[3] Acad Sci Res & Technol, ASRT, Cairo, Egypt

[4] Tallinn Univ Technol, ERA Chair Green Chem, Dept Chem & Biotechnol, Tallinn, Estonia

[5] City Sci Res & Technol Applicat CSAT, New Burg Al Arab Alexand, Egypt

来源：

JOURNAL OF SCIENTIFIC & INDUSTRIAL RESEARCH | 2020年 / 79卷 / 09期

关键词：

Probiotic cultivation; High cell mass production; Medium optimization; HELICOBACTER-PYLORI INFECTION; DOUBLE-BLIND; OPTIMIZATION; GROWTH; YEAST;

D O I：

暂无

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

Probiotics referred to a group of living microorganisms which highly influence the human health. A number of studies have highlighted on the bio-therapeutic potential of Lactobacillus reuteri strains, especially in treating eradication of Helicobacter pylori. Therefore, in present investigation, statistical methods were applied to optimize medium composition for high cell mass production of L. reuteri strain DSM 20016 T . Most influencing medium components were screened by using Plackett-Burman and optimized using Box-Bhenken experimental design. The concentration of lactose, yeast extract and phosphate in cultivation medium has shown significant effect on the cell mass production. The highest cell mass obtained after 48h incubation was 3.96 +/- 0.02 gL(-1) in RSM-optimized medium compared to 1.76 +/- 0.17 gL(-1) in un-optimized medium.

引用

页码：798 / 803

页数：6