Isolation and identification of Klebsiella aerogenes from bee colonies in bee dysbiosis

This article presents modern methods for obtaining and isolating pure culture from bee colonies (from honeycombs with infected broods and contaminated by faeces of bees), and the identification and indication of pathogenic bacteria of Klebsiella aerogenes species in honey bees during the winter, spring, summer and the autumn. The purpose was to isolate and identify the pure culture of enterobacteria from the pathological material of diseased bee colonies. The study of their basic biological properties for the possible use of the obtained strains, which should be used as indicator bacterial cultures in the study of the biological properties of drugs for the treatment and prevention of enterobacteriosis of bees was undertaken. The etiological factor for the origin of the collapse of bee colonies belongs to the Family Enterobacteriaceae, the genus being Klebsiella - Klebsiella aerogenes (previously named Enterobacter aerogenes), which was established by its cultural properties (Endo - light pink, small, flat, mucous, matte colonies; Levin - light pink, flat, mucous, matte colonies); by morphological properties (short, sticks, small, placed together and singly, without capsules, mobile); by tinctorial (gram negative) properties and by results of biochemical typing of microorganisms (Kligler medium: glucose - negative, acid-gas H2S negative; Simons medium - positive, acetate Na positive, malonate Na - negative; Phenylalanine - negative: Indole -negative; Urease - negative), which were isolated from the intestines of bees and their faeces from the frames of bee colonies.