Azobenzene-modified DNA aptamers evolved by capillary electrophoresis (CE)-SELEX method

被引：10

作者：

Morihiro, Kunihiko ^{[1
,2
,5
]}

Hasegawa, Osamu ^{[2
]}

Kasahara, Yuuya ^{[1
,2
]}

Mori, Shohei ^{[2
]}

Kasai, Tatsuro ^{[3
]}

Kuwahara, Masayasu ^{[3
,4
]}

Obika, Satoshi ^{[1
,2
]}

机构：

[1] Natl Inst Biomed Innovat Hlth & Nutr NIBIOHN, 7-6-8 Saito Asagi, Ibaraki, Osaka 5670085, Japan

[2] Osaka Univ, Grad Sch Pharmaceut Sci, 1-6 Yamadaoka, Suita, Osaka 5650871, Japan

[3] Gunma Univ, Grad Sch Sci & Technol, 1-5-1 Tenjin Cho, Kiryu, Gunma 3768515, Japan

[4] Nihon Univ, Grad Sch Integrated Basic Sci, Setagaya Ku, 3-25-40 Sakurajosui, Tokyo 1568550, Japan

[5] Univ Tokyo, Grad Sch Engn, Dept Chem & Biotechnol, Bunkyo Ku, 7-3-1 Hongo, Tokyo 1138656, Japan

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2021年 / 31卷

基金：

日本学术振兴会;

关键词：

Azobenzene; CE-SELEX; DNA aptamer; DNA polymerase; 5 '-Triphosphate; EQUILIBRIUM MIXTURES; EVOLUTION; BEARING;

D O I：

10.1016/j.bmcl.2020.127607

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Chemically modified aptamers have recently emerged as important materials for nucleic acid based therapeutics and diagnostic tools. Here, we report in vitro evolution of azobenzene-modified DNA aptamers by capillary electrophoresis (CE)-SELEX method. Azobenzene has been considered to be a fascinating functional group due to its trans-cis photo-isomerization property. We harnessed C5-azobenzene-modified 2'-deoxyuridine (dU(Az)) as a azobenzene-tethered unit and subjected it to CE-SELEX with human thrombin. The obtained dU(Az)-modified aptamer showed strong binding affinity toward human thrombin and could be reversibly photo-isomerized by different wavelengths of light. This work demonstrates that CE-SELEX is a powerful method to obtain chemically modified aptamers and dU(Az) is an excellent photo-responsive nucleoside for nucleic acid photo-switches.

引用

页数：4

共 21 条

[1] Photoisomerization in different classes of azobenzene
Bandara, H. M. Dhammika
Burdette, Shawn C.
[J]. CHEMICAL SOCIETY REVIEWS, 2012, 41 (05) : 1809 - 1825
[2] Azobenzene photoswitches for biomolecules
Beharry, Andrew A.
Woolley, G. Andrew
[J]. CHEMICAL SOCIETY REVIEWS, 2011, 40 (08) : 4422 - 4437
[3] Nonequilibrium capillary electrophoresis of equilibrium mixtures - A single experiment reveals equilibrium and kinetic parameters of protein-DNA interactions
Berezovski, M
Krylov, SN
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2002, 124 (46) : 13674 - 13675
[4] Light-Driven DNA Nanomachine with a Photoresponsive Molecular Engine
Kamiya, Yukiko
Asanuma, Hiroyuki
[J]. ACCOUNTS OF CHEMICAL RESEARCH, 2014, 47 (06) : 1663 - 1672
[5] Using photons to manipulate enzyme inhibition by an azobenzene-modified nucleic acid probe
Kim, Youngmi
Phillips, Joseph A.
Liu, Haipeng
Kang, Huaizhi
Tan, Weihong
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2009, 106 (16) : 6489 - 6494
[6] Molecular Evolution of Functional Nucleic Acids with Chemical Modifications
Kuwahara, Masayasu
Sugimoto, Naoki
[J]. MOLECULES, 2010, 15 (08) : 5423 - 5444
[7] In vitro selection of a photoresponsive RNA aptamer to hemin
Liu, Mingzhe
Jinmei, Hiroshi
Abe, Hiroshi
Ito, Yoshihiro
[J]. BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 2010, 20 (09) : 2964 - 2967
[8] Sialyllactose-binding modified DNA aptamer bearing additional functionality by SELEX
Masud, MM
Kuwahara, M
Ozaki, H
Sawai, H
[J]. BIOORGANIC & MEDICINAL CHEMISTRY, 2004, 12 (05) : 1111 - 1120
[9] The Chemical Biology of Aptamers
Mayer, Guenter
[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2009, 48 (15) : 2672 - 2689
[10] In vitro evolution of functional DNA using capillary electrophoresis
Mendonsa, SD
Bowser, MT
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (01) : 20 - 21

← 1 2 3 →