Skin Fibroblast Cells on 3D Skin Cell Chip Using Nanogold Platform Structures and Three-Floor Structures

A novel, three-dimensional (3D) skin cell chip was developed using a three-floor structure and a nanogold platform structure cell chip for the ex vivo 3D cell growth simulation of skin cells and to evaluate the efficacy of the system for detection of cosmetic drugs. The nanogold platform structures were fabricated through a nanoporous alumina mask, and the structural formations were confirmed via scanning electron microscopy. A three-floor structure cell chip was fabricated using a two-photon stereolithography process. The skin cells were attached to RGD (Arg-Gly-Asp) peptide nanopatterned surfaces, and electrochemical tools were applied to the skin cells attached to the chip surface. Treatment with imidazolidinyl urea induced a loss of cell viability in a concentration-dependent manner using the three-floor structure cell chip and nanogold structured cell chip. The observed increased chemoresistance to cosmetic drugs in the three-floor structure cell chip might result from the effects of cell confluence. Furthermore, these results strongly indicate that this new cell array system is suitable for high-throughput cytotoxicity and genotoxicity screening applications.