The genetic transformation machinery: composition, localization, and mechanism

被引：176

作者：

Claverys, Jean-Pierre ^{[1
,2
]}

Martin, Bernard ^{[2
]}

Polard, Patrice ^{[2
]}

机构：

[1] Univ Toulouse 3, Univ Toulouse, LMGM, CNRS, F-31062 Toulouse 9, France

[2] LMGM, UMR5100, CNRS, Toulouse, France

来源：

FEMS MICROBIOLOGY REVIEWS | 2009年 / 33卷 / 03期

关键词：

transformation; recombination; recombination mediator protein; DNA uptake machine; DNA processing machine; DprA; SINGLE-STRANDED-DNA; COMPETENT BACILLUS-SUBTILIS; TRANSCRIPTION FACTOR COMK; GRAM-POSITIVE BACTERIA; COLI RECO PROTEIN; STREPTOCOCCUS-PNEUMONIAE; BINDING-PROTEIN; HOMOLOGOUS RECOMBINATION; DEOXYRIBONUCLEIC-ACID; ECLIPSE COMPLEX;

D O I：

10.1111/j.1574-6976.2009.00164.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Natural genetic transformation is widely distributed in bacteria. It is a genetically programmed process that is inherent to the species. Transformation requires a specialized membrane-associated machinery for uptake of exogenous double-stranded DNA. It also requires dedicated cytosolic proteins, some of which have been characterized only recently, for the processing of internalized single-stranded DNA fragments into recombination products. A series of observations made in Bacillus subtilis and Streptococcus pneumoniae led to the recent emergence of a picture of a unique, highly integrated machine localized at the cell poles. This dynamic machine, which we propose to name the transformasome, involves both membrane and cytosolic proteins, to internalize, protect, and process transforming DNA. This review attempts to summarize these recent observations with special emphasis on the early stages in DNA processing.

引用

页码：643 / 656

页数：14

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