Bone marrow-derived dendritic cells under influence of experimental breast cancer and physical activity

Immune cells are required in the immune response against tumours, although sometimes without success. The present study aimed to investigate dendritic cell (DC) maturation in animals with induced immunosuppression that were subjected to physical activity (PA). Immunosuppression was induced using 7,12-dimethyl-benzanthracene (DMBA). A total of 56 Balb/c mice were divided into four groups, including the control group, non-DMBA administered/PA group (GII), DMBA administered/non-PA group (GIII) and the DMBA administered/PA group (GIV). Bone marrow was removed from the leg bones following sacrifice. Bone marrow-derived DCs were stimulated to differentiate by granulocytemacro-phage colony-stimulating factor, interleukin (IL)-4 and tumour necrosis factor-alpha, after which the phenotype was assessed by flow cytometry and the cytokine profile was assessed using ELISAs. PA significantly increased the percentage of DCs in GII (55.38 +/- 2.63%) and GIV (50.1 +/- 3.1%) mice, as compared with GI (34.61 +/- 1.28%) and GIII (36.25 +/- 1.85%) mice (P<0.05). In addition, GIV mice showed a significantly higher level of cluster of differentiation (CD) 80(+)/CD86(+) DCs (76.38 +/- 6.31%), as compared with GI (54.03 +/- 6.52%) and GIII (52.07 +/- 5.74%) mice (P<0.05). Furthermore, GIV mice showed a significantly higher level of CD80+/major histocompatibility complex class II double labelling (P<0.05), as compared with GIV (95.35 +/- 1.22%) and GIII (76.15 +/- 5.53%) mice. The expression of interferon-gamma was significantly increased in GIV mice [5.89 (5.2-7.12)], as compared with GIII mice [2.75 (1.33-4.4)] (P<0.05). Similarly, the expression of IL-12 was markedly increased in GIV mice [1.27 (0.26-2.57)] compared with GIII mice [0.73 (0.44-1.47)], although the difference was not significant (P=0.063). The results of the present study suggested that PA was able to promote the maturation of DCs and their secretion of anti-tumour cytokines. Therefore, PA may emerge as a tool in immunotherapy.