Self-Assembling Peptide Nanofibrous Hydrogel on Immediate Hemostasis and Accelerative Osteosis

被引：43
作者：
Wu, Min [1 ,2 ]
Ye, Zhaoyang [2 ]
Zhu, Hongyan [3 ]
Zhao, Xiaojun [2 ,4 ]
机构：
[1] Sichuan Univ, West China Hosp, HMRRC, Dept Radiol, Chengdu 610041, Sichuan, Peoples R China
[2] Sichuan Univ, West China Hosp, Inst Nanobiomed Technol & Membrane Biol, Chengdu 610041, Sichuan, Peoples R China
[3] Sichuan Univ, West China Hosp, State Key Lab Biotherapy, Lab Stem Cell Biol, Chengdu 610041, Sichuan, Peoples R China
[4] MIT, Ctr Biomed Engn, NE47 379, Cambridge, MA 02139 USA
来源：
BIOMACROMOLECULES | 2015年 / 16卷 / 10期
基金：
中国国家自然科学基金;
关键词：
BONE WAX; COMPLEMENTARY OLIGOPEPTIDE; REGENERATIVE MEDICINE; PROGENITOR CELLS; SPINAL-CORD; BETA-SHEET; IN-VIVO; SCAFFOLDS; DESIGN; DIFFERENTIATION;
D O I：
10.1021/acs.biomac.5b00493
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The use of local agents to achieve hemostasis of bone that does not interfere with repair and recovery is a complex and emergency subject in surgery. In this study, the dual functional biodegradable self-assembling nanopeptide (SAP) RADA16-I was synthesized by solid phase synthesis and was shown to exhibit immediate hemostasis and accelerative osteosis. The RADA16-I showed good performance as a hemostatic agent, which was investigated by comparison with the effects of bone wax in the ilium bone defect model of New Zealand rabbits. The RADA16-I exhibited efficient function of bone regeneration in both radiographic analysis and histological examination, while the bone wax inhibited osteogenesis. Moreover, in in vivo experiment, the RADA16-I was shown to exhibit excellent biocompatibility, while the group with bone wax showed a severe inflammatory response at the interface with bone. Thus, the RADA16-I is proven to be an excellent biocompatible material with effective dual function of hemostasis and osteosis.
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收藏
页码：3112 / 3118
页数：7
相关论文
共 49 条
[31]   PuraMatrix™ facilitates bone regeneration in bone defects of calvaria in mice [J].
Misawa, Haruo ;
Kobayashi, Naoya ;
Soto-Gutierrez, Alejandro ;
Chen, Yong ;
Yoshida, Aki ;
Rivas-Carrillo, Jorge David ;
Navarro-Alvarez, Nalu ;
Tanaka, Kirniaki ;
Miki, Atsushi ;
Takei, Jiro ;
Ueda, Tadayoshi ;
Tanaka, Masato ;
Endo, Hirosuke ;
Tanaka, Noriaki ;
Ozaki, Toshifumi .
CELL TRANSPLANTATION, 2006, 15 (10) :903-910
[32]   BD PuraMatrix peptide hydrogel as a culture system for human fetal Schwann cells in spinal cord regeneration [J].
Moradi, Fateme ;
Bahktiari, Mehrdad ;
Joghataei, Mohammad Taghi ;
Nobakht, Maliheh ;
Soleimani, Masoud ;
Hasanzadeh, Gholamreza ;
Fallah, Ali ;
Zarbakhsh, Sam ;
Hejazian, Leila Beigom ;
Shirmohammadi, Maryam ;
Maleki, Fatemeh .
JOURNAL OF NEUROSCIENCE RESEARCH, 2012, 90 (12) :2335-2348
[33]   A specific heptapeptide from a phage display peptide library homes to bone marrow and binds to primitive hematopoietic stem cells [J].
Nowakowski, GS ;
Dooner, MS ;
Valinski, HM ;
Mihaliak, AM ;
Quesenberry, PJ ;
Becker, PS .
STEM CELLS, 2004, 22 (06) :1030-1038
[34]   Applications of self-assembling peptide scaffolds in regenerative medicine: the way to the clinic [J].
Ravichandran, Ranjithkumar ;
Griffith, May ;
Phopase, Jaywant .
JOURNAL OF MATERIALS CHEMISTRY B, 2014, 2 (48) :8466-8478
[35]  
Schmitz JP., 1986, CLIN ORTHOP RELAT R, V205, P299
[36]   The use of local agents: bone wax, gelatin, collagen, oxidized cellulose [J].
Schonauer, C ;
Tessitore, E ;
Barbagallo, G ;
Albanese, V ;
Moraci, A .
EUROPEAN SPINE JOURNAL, 2004, 13 (Suppl 1) :S89-S96
[37]   Entrapment of migrating hippocampal neural cells in three-dimensional peptide nanofiber scaffold [J].
Semino, CE ;
Kasahara, J ;
Hayashi, Y ;
Zhang, SG .
TISSUE ENGINEERING, 2004, 10 (3-4) :643-655
[38]   Functional differentiation of hepatocyte-like spheroid structures from putative liver progenitor cells in three-dimensional peptide scaffolds [J].
Semino, CE ;
Merok, JR ;
Crane, GG ;
Panagiotakos, G ;
Zhang, SG .
DIFFERENTIATION, 2003, 71 (4-5) :262-270
[39]  
Soler-Botija C, 2014, AM J TRANSL RES, V6, P291
[40]   Ostene, a new water-soluble bone hemostasis agent [J].
Tadeusz, Wellisz ;
Armstrong, Jonathan K. ;
Cambridge, John ;
Fisher, Timothy C. .
JOURNAL OF CRANIOFACIAL SURGERY, 2006, 17 (03) :420-425
12345