Spying the neutralizing epitopes on E2 N-terminal by candidate epitope-vaccines against classical swine fever virus

被引：26
作者：
Dong, XN [1 ]
Chen, YH [1 ]
机构：
[1] Tsing Hua Univ, Immunol Lab, Dept Biol, Prot Sci Lab,Minist Educ, Beijing 100084, Peoples R China
来源：
VACCINE | 2006年 / 24卷 / 19期
关键词：
classical swine fever virus (CSFV); sequential neutralizing epitope; epitope-vaccine; marker vaccine;
D O I：
10.1016/j.vaccine.2006.01.003
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Our previous study proved that the N-terminal (aa693-711) of glycoprotein E2 contained sequential neutralizing epitopes. In this study, four candidate epitope-vaccines (EVs) were separately prepared and evaluated. Among them, epitope-vaccine EV-BC1a (BC1a: aa693-699) induced high level of epitope-specific neutralizing antibodies and exhibited similar protective capability with that induced by Chinese vaccine strain (C-strain). These results confirmed CKEDYRY (aa693-699) as a principal sequential neutralizing epitope on E2 N-terminal. Moreover, these findings also indicate that epitope-vaccine is a potent candidate strategy for marker vaccine against classical swine fever virus (CSFV). (c) 2006 Elsevier Ltd. All rights reserved.
引用
收藏
页码：4029 / 4034
页数：6
相关论文
共 22 条
[1]   Prototype Alzheimer's disease vaccine using the immunodominant B cell epitope from β-amyloid and promiscuous T cell epitope pan HLA DR-binding peptide [J].
Agadjanyan, MG ;
Ghochikyan, A ;
Petrushina, I ;
Vasilevko, V ;
Movsesyan, N ;
Mkrtichyan, M ;
Saing, T ;
Cribbs, DH .
JOURNAL OF IMMUNOLOGY, 2005, 174 (03) :1580-1586
[2]   Epitope vaccine: a new strategy against HIV-1 [J].
Chen, YH ;
Xiao, Y ;
Yu, TW ;
Dierich, MP .
IMMUNOLOGY TODAY, 1999, 20 (12) :588-589
[3]   Chimeric (marker) C-strain viruses induce clinical protection against virulent classical swine fever virus (CSFV) and reduce transmission of CSFV between vaccinated pigs [J].
de Smit, AJ ;
Bouma, A ;
van Gennip, HGP ;
de Kluijver, EP ;
Moormann, RJM .
VACCINE, 2001, 19 (11-12) :1467-1476
[4]   Recombinant classical swine fever (CSF) viruses derived from the Chinese vaccine strain (C-strain) of CSF virus retain their avirulent and immunogenic characteristics [J].
de Smit, AJ ;
van Gennip, HGP ;
Miedema, GKW ;
van Rijn, PA ;
Terpstra, C ;
Moormann, RJM .
VACCINE, 2000, 18 (22) :2351-2358
[5]   The neutralizing epitope ELDKWA on HIV-1 gp41: Genetic variability and antigenicity [J].
Dong, XN ;
Wu, Y ;
Chen, YH .
IMMUNOLOGY LETTERS, 2005, 101 (01) :81-86
[6]   Candidate multi-peptide-vaccine against classical swine fever virus induced potent immunity with serological marker [J].
Dong, XN ;
Chen, Y ;
Wu, Y ;
Chen, YH .
VACCINE, 2005, 23 (28) :3630-3633
[7]   Candidate peptide vaccine induced protection against classical swine fever virus [J].
Dong, XN ;
Wei, K ;
Liu, ZQ ;
Chen, YH .
VACCINE, 2002, 21 (3-4) :167-173
[8]   Candidate peptide-vaccine induced potent protection against CSFV and identified a principal sequential neutralizing determinant on E2 [J].
Dong, XN ;
Qi, Y ;
Ying, R ;
Chen, X ;
Chen, YH .
VACCINE, 2006, 24 (04) :426-434
[9]  
DONG XN, VACCINE
[10]   Parenteral and mucosal delivery of a novel multi-epitope M protein-based group A streptococcal vaccine construct: investigation of immunogenicity in mice [J].
Dunn, LA ;
McMillan, DJ ;
Batzloff, M ;
Zeng, WG ;
Jackson, DCJ ;
Upcroft, JA ;
Upcroft, P ;
Olive, C .
VACCINE, 2002, 20 (21-22) :2635-2640
123