Isolation of cyanidin 3-glucoside from blue honeysuckle fruits by high-speed counter-current chromatography

被引：44

作者：

Chen, Liang ^{[1
]}

Xin, Xiulan ^{[1
]}

Lan, Rong ^{[1
]}

Yuan, Qipeng ^{[2
]}

Wang, Xiaojie ^{[1
]}

Li, Ye ^{[1
]}

机构：

[1] Beijing Polytech, Coll Bioengn, Beijing 100029, Peoples R China

[2] Beijing Univ Chem Technol, State Key Lab Chem Resource Engn, Beijing 100029, Peoples R China

来源：

FOOD CHEMISTRY | 2014年 / 152卷

关键词：

Cyanidin; 3-glucoside; Blue honeysuckle fruits; High-speed counter-current; chromatography (HSCCC); ANTIOXIDANT ACTIVITY; LONICERA-CAERULEA; SOLVENT SYSTEM; ANTHOCYANINS; PURIFICATION;

D O I：

10.1016/j.foodchem.2013.11.080

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Blue honeysuckle fruits are rich in anthocyanins with many beneficial effects such as reduction of the risk of cardiovascular diseases, diabetes and cancers. High-speed counter-current chromatography (HSCCC) was used for the separation of anthocyanin on a preparative scale from blue honeysuckle fruit crude extract with a biphasic solvent system composed of tert-butyl methyl ether/n-butanol/acetonitrile/ water/trifluoroacetic acid (2:2:1:5:0.01, v/v) for the first time in this paper. Each injection of 100 mg crude extract yielded 22.8 mg of cyanidin 3-glucoside (C3G) at 98.1% purity. The compound was identified by means of electro-spray ionisation mass (ESI/MS) and 1H and 13C nuclear magnetic resonance (NMR) spectra.(C) 2013 Elsevier Ltd. All rights reserved.

引用

页码：386 / 390

页数：5

共 27 条

[1]

AOKI H., 2002, Foods and Food Ingredients Journal of Japan, P41

[2] Characterization and quantification of Anthocyanins and polyphenolics in blue honeysuckle (Lonicera caerulea L.) [J].

Chaovanalikit, A ;

Thompson, MM ;

Wrolstad, RE .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2004, 52 (04) :848-852

[3] Black rice anthocyanins inhibit cancer cells invasion via repressions of MMPs and u-PA expression [J].

Chen, Pei-Ni ;

Kuo, Wu-Hsien ;

Chiang, Chui-Liang ;

Chiou, Hui-Ling ;

Hsieh, Yih-Shou ;

Chu, Shu-Chen .

CHEMICO-BIOLOGICAL INTERACTIONS, 2006, 163 (03) :218-229

[4] Separation and purification of anthocyanins by high-speed countercurrent chromatography and screening for antioxidant activity [J].

Degenhardt, A ;

Knapp, H ;

Winterhalter, P .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2000, 48 (02) :338-343

[5] Composition of anthocyanins in mulberry and their antioxidant activity [J].

Du, Q. ;

Zheng, J. ;

Xu, Y. .

JOURNAL OF FOOD COMPOSITION AND ANALYSIS, 2008, 21 (05) :390-395

[6] Counter-current chromatography: instrumentation, solvent selection and some recent applications to natural product purification [J].

Foucault, AP ;

Chevolot, L .

JOURNAL OF CHROMATOGRAPHY A, 1998, 808 (1-2) :3-22

[7] Rational development of solvent system families in counter-current chromatography [J].

Friesen, J. Brent ;

Pauli, Guido F. .

JOURNAL OF CHROMATOGRAPHY A, 2007, 1151 (1-2) :51-59

[8]

Ghosh D, 2007, ASIA PAC J CLIN NUTR, V16, P200

[9] A simple method to optimize the HSCCC two-phase solvent system by predicting the partition coefficient for target compound [J].

Han, Quan-Bin ;

Wong, Lina ;

Yang, Nian-Yun ;

Song, Jing-Zheng ;

Qiao, Chun-Feng ;

Yiu, Hillary ;

Ito, Yoichiro ;

Xu, Hong-Xi .

JOURNAL OF SEPARATION SCIENCE, 2008, 31 (6-7) :1189-1194

[10] Golden rules and pitfalls in selecting optimum conditions for high-speed counter-current chromatography [J].

Ito, Y .

JOURNAL OF CHROMATOGRAPHY A, 2005, 1065 (02) :145-168

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