Is selenoprotein K required for Borrelia burgdorferi infection within the tick vector Ixodes scapularis?

被引:10
作者
Kumar, Deepak [1 ]
Embers, Monica [2 ]
Mather, Thomas N. [3 ]
Karim, Shahid [1 ]
机构
[1] Univ Southern Mississippi, Dept Cell & Mol Biol, Sch Biol Environm & Earth Sci, Hattiesburg, MS 39406 USA
[2] Tulane Natl Primate Res Ctr, Div Immunol, Covington, LA 70455 USA
[3] Univ Rhode Isl, Ctr Vector Borne Dis, Kingston, RI 02881 USA
关键词
Tick; Ixodes scapularis; Borrelia burgdorferi; Lyme disease; Selenoproteins; ER stress; ENDOPLASMIC-RETICULUM; AMBLYOMMA-MACULATUM; RICKETTSIA-PARKERI; PATHOGEN; PROTEIN; ACARI; HOST; COLONIZATION; ACTIVATION; BINDING;
D O I
10.1186/s13071-019-3548-y
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
BackgroundTick selenoproteins are involved in regulating oxidative and endoplasmic reticulum stress during prolonged tick feeding on mammalian hosts. How selenoproteins are activated upon tick-borne pathogen infection is yet to be defined.MethodsTo examine the functional role of selenoprotein K in Borrelia burgdorferi infection within the tick host Ixodes scapularis, RNA interference (RNAi)-based gene silencing was performed.ResultsSelenoprotein K is an endoplasmic reticulum (ER)-resident protein and a component of the ERAD complex involved in ER homeostasis. A qRT-PCR assay revealed the significant upregulation of selenogene K (selenoK) expression in B. burgdorferi-infected tick tissues. Silencing of the selenoK transcript significantly depleted B. burgdorferi copies within the infected tick tissues. Upon selenoK knockdown, another component of the ERAD complex, selenoprotein S (selenoS), was significantly upregulated, suggesting a compensatory mechanism to maintain ER homeostasis within the tick tissues. Knockdown of selenoK also upregulated ER stress-related unfolded protein response (UPR) pathway components, ATF6 and EIF2.ConclusionsThe exact mechanisms that contribute to depletion of B. burgdorferi upon selenoK knockdown is yet to be determined, but this study suggests that selenoK may play a vital role in the survival of B. burgdorferi within the tick host.
引用
收藏
页数:8
相关论文
共 50 条
[41]   Phylogeographic dynamics of the arthropod vector, the blacklegged tick (Ixodes scapularis) [J].
O'Keeffe, Kayleigh R. ;
Oppler, Zachary J. ;
Prusinski, Melissa ;
Falco, Richard C. ;
Oliver, JoAnne ;
Haight, Jamie ;
Sporn, Lee Ann ;
Backenson, P. Bryon ;
Brisson, Dustin .
PARASITES & VECTORS, 2022, 15 (01)
[42]   Geographic distribution of ticks (Acari:Ixodidae) in Iowa with emphasis on Ixodes scapularis and their infection with Borrelia burgdorferi [J].
Lingren, M ;
Rowley, WA ;
Thompson, C ;
Gilchrist, M .
VECTOR-BORNE AND ZOONOTIC DISEASES, 2005, 5 (03) :219-226
[43]   Vector competence of the blacklegged tick, Ixodes scapularis, for the recently recognized Lyme borreliosis spirochete Candidatus Borrelia mayonii [J].
Dolan, Marc C. ;
Hojgaard, Andrias ;
Hoxmeier, J. Charles ;
Replogle, Adam J. ;
Respicio-Kingrya, Laurel B. ;
Sexton, Christopher ;
Williams, Martin A. ;
Pritt, Bobbi S. ;
Schriefer, Martin E. ;
Eisen, Lars .
TICKS AND TICK-BORNE DISEASES, 2016, 7 (05) :665-669
[44]   Deer management generally reduces densities of nymphal Ixodes scapularis, but not prevalence of infection with Borrelia burgdorferi sensu stricto [J].
Martin, Alynn M. ;
Buttke, Danielle ;
Raphael, Jordan ;
Taylor, Kelsey ;
Maes, Sarah ;
Parise, Christina M. ;
Ginsberg, Howard S. ;
Cross, Paul C. .
TICKS AND TICK-BORNE DISEASES, 2023, 14 (05)
[45]   Mapping distributions of the Lyme disease vector, Ixodes scapularis, and spirochete, Borrelia burgdorferi, in Kentucky using passive and active surveillance [J].
Pasternak, Anna R. ;
Palli, Subba R. .
TICKS AND TICK-BORNE DISEASES, 2022, 13 (02)
[46]   RANGE EXPANSION OF IXODES SCAPULARIS TICKS AND OF BORRELIA BURGDORFERI BY MIGRATORY BIRDS [J].
Heffernan, Jane M. ;
Lou, Yijun ;
Wu, Jianhong .
DISCRETE AND CONTINUOUS DYNAMICAL SYSTEMS-SERIES B, 2014, 19 (10) :3147-3167
[47]   Distribution and characterization of Borrelia burgdorferi isolates from Ixodes scapularis and presence in mammalian hosts in Ontario, Canada [J].
Morshed, M. G. ;
Scott, J. D. ;
Fernando, K. ;
Geddes, G. ;
McNabb, A. ;
Mak, S. ;
Durden, L. A. .
JOURNAL OF MEDICAL ENTOMOLOGY, 2006, 43 (04) :762-773
[48]   Genotypic Diversity of an Emergent Population of Borrelia burgdorferi at a Coastal Maine Island Recently Colonized by Ixodes scapularis [J].
MacQueen, Douglas D. ;
Lubelczyk, Charles ;
Elias, Susan P. ;
Cahill, Bruce K. ;
Mathers, Amy J. ;
Lacombe, Eleanor H. ;
Rand, Peter W. ;
Smith, Robert P., Jr. .
VECTOR-BORNE AND ZOONOTIC DISEASES, 2012, 12 (06) :456-461
[49]   Characterization of Anaplasma phagocytophila and Borrelia burgdorferi genotypes in Ixodes scapularis ticks from Pennsylvania [J].
Courtney, JW ;
Dryden, RL ;
Wyleto, P ;
Schneider, BS ;
Massung, RF .
RICKETTSIOLOGY: PRESENT AND FUTURE DIRECTIONS, 2003, 990 :131-133
[50]   Tick-Tattoo: DNA Vaccination Against B. burgdorferi or Ixodes scapularis Tick Proteins [J].
Klouwens, Michelle J. ;
Trentelman, Jos J. A. ;
Wagemakers, Alex ;
Ersoz, Jasmin, I ;
Bins, Adriaan D. ;
Hovius, Joppe W. .
FRONTIERS IN IMMUNOLOGY, 2021, 12