Is selenoprotein K required for Borrelia burgdorferi infection within the tick vector Ixodes scapularis?

被引：10

作者：

Kumar, Deepak ^{[1
]}

Embers, Monica ^{[2
]}

Mather, Thomas N. ^{[3
]}

Karim, Shahid ^{[1
]}

机构：

[1] Univ Southern Mississippi, Dept Cell & Mol Biol, Sch Biol Environm & Earth Sci, Hattiesburg, MS 39406 USA

[2] Tulane Natl Primate Res Ctr, Div Immunol, Covington, LA 70455 USA

[3] Univ Rhode Isl, Ctr Vector Borne Dis, Kingston, RI 02881 USA

来源：

PARASITES & VECTORS | 2019年 / 12卷 / 1期

关键词：

Tick; Ixodes scapularis; Borrelia burgdorferi; Lyme disease; Selenoproteins; ER stress; ENDOPLASMIC-RETICULUM; AMBLYOMMA-MACULATUM; RICKETTSIA-PARKERI; PATHOGEN; PROTEIN; ACARI; HOST; COLONIZATION; ACTIVATION; BINDING;

D O I：

10.1186/s13071-019-3548-y

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

BackgroundTick selenoproteins are involved in regulating oxidative and endoplasmic reticulum stress during prolonged tick feeding on mammalian hosts. How selenoproteins are activated upon tick-borne pathogen infection is yet to be defined.MethodsTo examine the functional role of selenoprotein K in Borrelia burgdorferi infection within the tick host Ixodes scapularis, RNA interference (RNAi)-based gene silencing was performed.ResultsSelenoprotein K is an endoplasmic reticulum (ER)-resident protein and a component of the ERAD complex involved in ER homeostasis. A qRT-PCR assay revealed the significant upregulation of selenogene K (selenoK) expression in B. burgdorferi-infected tick tissues. Silencing of the selenoK transcript significantly depleted B. burgdorferi copies within the infected tick tissues. Upon selenoK knockdown, another component of the ERAD complex, selenoprotein S (selenoS), was significantly upregulated, suggesting a compensatory mechanism to maintain ER homeostasis within the tick tissues. Knockdown of selenoK also upregulated ER stress-related unfolded protein response (UPR) pathway components, ATF6 and EIF2.ConclusionsThe exact mechanisms that contribute to depletion of B. burgdorferi upon selenoK knockdown is yet to be determined, but this study suggests that selenoK may play a vital role in the survival of B. burgdorferi within the tick host.

引用

页数：8