Detecting protein-protein interactions by Xe-129 NMR

被引：7

作者：

Zhao, Zhuangyu ^{[1
]}

Roose, Benjamin W. ^{[1
]}

Zemerov, Serge D. ^{[1
]}

Stringer, Madison A. ^{[1
]}

Dmochowski, Ivan J. ^{[1
]}

机构：

[1] Univ Penn, Dept Chem, Philadelphia, PA 19104 USA

来源：

CHEMICAL COMMUNICATIONS | 2020年 / 56卷 / 75期

关键词：

FRAGMENT COMPLEMENTATION; PICOMOLAR SENSITIVITY; LIVING CELLS; IN-VIVO; C-FOS; REPORTER; XENON; BINDING; CRYPTOPHANE; INHIBITORS;

D O I：

10.1039/d0cc02988b

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Detection of protein-protein interactions (PPIs) is limited by current bioanalytical methods. A protein complementation assay (PCA), split TEM-1 beta-lactamase, interacts with xenon at the interface of the TEM-1 fragments. Reconstitution of TEM-1-promoted here by cFos/cJun leucine zipper interaction-gives rise to sensitive(129)Xe NMR signal in bacterial cells.

引用

页码：11122 / 11125

页数：4

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