Possible Effects of Interferon-α on Fas-Induced Renal Apoptosis in Mouse

被引：0

作者：

Cvetkovic, T. ^{[1
]}

Kocic, G. ^{[1
]}

Jevtovic-Stoimenov, T. ^{[1
]}

Pavlovic, D. ^{[1
]}

Basic, J. ^{[1
]}

Topalovic, A. ^{[1
]}

Veljkovic, A. ^{[1
]}

机构：

[1] Univ Nis, Inst Biochem, Fac Med, Nish 18000, Serbia

来源：

RENAL FAILURE | 2009年 / 31卷 / 05期

关键词：

Fas; apoptosis; oxidative stress; interferon-alpha; MEDIATED APOPTOSIS; CELLS; MACROPHAGES; INDUCTION; KIDNEY; INJURY; LIGAND; MICE;

D O I：

10.1080/08860220902882030

中图分类号：

R5 [内科学]; R69 [泌尿科学（泌尿生殖系疾病）];

学科分类号：

1002 ; 100201 ;

摘要：

Fas (APO-1/CD95) is a cell surface receptor that initiates apoptotic pathway. Fas-stimulated ROS generation may play important role in Fas-mediated apoptosis. The aim of this study was to evaluate the influence of interferon-alpha on oxidative stress parameters in Fas-induced renal apoptosis in mice kidney. Subjects and methods. One-month-old Balb C male mice were used for the study. The animals were divided in four groups: group I were the controls, group 2 mice were treated with anti-Fas antibody i.p., group 3 mice were treated wit IFN-alpha, and group 4 mice were treated with both agents simultaneously. The mice were killed 48 h afterwards, and kidneys were homogenized. TBA reactive substances (TBARS), glutathione content, and reactive carbonyl group (RCG) were measured. Results. The results showed a statistically significant increase of TBARS (p < 0.05) and RCG (p < 0.05) concentration in the group treated with anti-Fas antibody versus control. IFN-a decreased the concentration of TBARS and RCG after anti-Fas antibody administration (p < 0.05). There is no significant difference in glutathione content between investigated groups. Conclusion. IFN-alpha might be considered as a new target for therapeutic intervention in FasL/Fas induced renal injury.

引用

页码：377 / 381

页数：5

共 21 条

[1] TISSUE SULFHYDRYL GROUPS [J].