Comparison of the development of mouse embryos manipulated with different biopsy techniques

被引：6

作者：

Taskin, Ali Cihan ^{[1
]}

Akkoc, Tolga ^{[2
]}

Sagirkaya, Hakan ^{[3
]}

Bagis, Haydar ^{[4
]}

Arat, Sezen ^{[5
]}

机构：

[1] Koc Univ, Coll Sci, Istanbul, Turkey

[2] TUBITAK Marmara Res Ctr, Genet Engn & Biotechnol Inst, Reprod Biol Lab, Kocaeli, Turkey

[3] Uludag Univ, Dept Reprod & Artificial Inseminat, Fac Vet Med, Bursa, Turkey

[4] Adiyaman Univ, Dept Genet, Fac Med, Adiyaman, Turkey

[5] Namik Kemal Univ, Dept Biotechnol, Fac Agr, Tekirdag, Turkey

来源：

TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES | 2016年 / 40卷 / 02期

关键词：

Mouse; embryo; biopsy; blastomere; microblade; trophectoderm; PREIMPLANTATION GENETIC DIAGNOSIS; TROPHECTODERM BIOPSY; BLASTOMERES; PCR;

D O I：

10.3906/vet-1506-63

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Preimplantation genetic diagnosis is the detection of inherited diseases and the sex of embryos before implantation in the practice of human medicine as well as in veterinary medicine. The introduction of experimental animal embryo biopsy techniques has been a milestone in the developmental process of preimplantation genetic diagnosis techniques. The aim of the present study was to evaluate in vivo and in vitro development of embryos after biopsy in an experimental mouse model and to perform comparisons across different biopsy techniques (blastomere biopsy and trophectoderm biopsy). At the end of the study, no significant difference was observed between the blastomere biopsy group and the control group in terms of in vitro development, embryo quality, and fetal development, whereas embryo quality and in vivo development were negatively affected in the trophectoderm biopsy group (P < 0.05).

引用

页码：157 / 162

页数：6

共 23 条

[1] Comparison of Different Cryopreservation Techniques: Higher Survival and Implantation Rate of Frozen-Thawed Mouse Pronuclear Embryos in the Presence of Beta-Mercaptoethanol in Post-Thaw Culture [J].