Effect of extraction method on structure and antioxidant activity of Hohenbuehelia serotina polysaccharides

被引：114
作者：
Li, Xiaoyu [1 ,2 ]
Wang, Lu [1 ,2 ]
机构：
[1] Yanshan Univ, Sch Environm & Chem Engn, Qinhuangdao 066004, Peoples R China
[2] Harbin Inst Technol, Sch Food Sci & Engn, Harbin 150090, Peoples R China
来源：
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2016年 / 83卷
基金：
中国国家自然科学基金;
关键词：
Hohenbuehelia serotina; Polysaccharides; Extraction method; Preliminary structure; Antioxidant activity; ULTRASONIC-ASSISTED EXTRACTION; RESPONSE-SURFACE METHODOLOGY; ENZYMATIC EXTRACTION; OPTIMIZATION;
D O I：
10.1016/j.ijbiomac.2015.11.060
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The impacts of four extraction methods (hot water, enzyme assistance, ultrasonic assistance and ultrasonic-enzyme assistance) on the extraction yields, preliminary structure and antioxidant activities of the Hohenbuehelia serotina polysaccharides (HW-HSP, EA-HSP, UA-HSP and UEA-HSP) were systematically investigated. The yield of the polysaccharides (20.70 +/- 0.17%) obtained by ultrasonic-enzyme assistance was higher than the polysaccharides by other methods'. Four kinds of polysaccharides possessed the different preliminary structural characteristics including molecular weight distributions, monosaccharide compositions, crystallization and spiral structures, while different surface morphology. Through the measurements of antioxidant activities in vitro, UEA-HSP exhibited the most significant scavenging capacities on non-physiological ABTS free radicals and physiological hydroxyl radicals. These data showed that ultrasonic-enzyme assistance was more beneficial to enhance the extraction yields of the polysaccharides, and obtain higher bioactive polysaccharides. The results also suggested that H. serotina polysaccharides possessed potential healthcare application in food field due to their antioxidant activities. (C) 2015 Elsevier B.V. All rights reserved.
引用
收藏
页码：270 / 276
页数：7
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