Non-contact measurement of adsorbed cytochrome c with optical waveguide spectrometry;: The effect of distance between waveguide and protein on the spectral sensitivity

被引：17

作者：

Ohno, H ^{[1
]}

Fukuda, K ^{[1
]}

Kurusu, F ^{[1
]}

机构：

[1] Tokyo Univ Agr & Technol, Dept Biotechnol, Tokyo 1848588, Japan

来源：

CHEMISTRY LETTERS | 2000年 / 01期

关键词：

D O I：

10.1246/cl.2000.76

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Optical waveguide spectrometry with non-contact conditions was performed with the aim of widening the choice of substrate material. The visible absorption spectrum of cytochrome c (cyt.c) adsorbed on a quartz glass or gold surface was detected with the evanescent wave from a co-faced waveguide. A clear correlation was observed between absorbance of cyt.c and the gap between the waveguide surface and the protein.

引用

收藏

页码：76 / 77

页数：2

相关论文

共 12 条

[1]

ANDRADE JD, 1986, ADV POLYM SCI, V79, P1

[2] Regenerable biosensor platform: A total internal reflection fluorescence cell with electrochemical control [J].

Asanov, AN ;

Wilson, WW ;

Odham, PB .

ANALYTICAL CHEMISTRY, 1998, 70 (06) :1156-1163

[3] FTIR-ATR and radiolabeling study of the adsorption of ribonuclease A onto hydrophilic surfaces: Correlation between the exchange rate and the interfacial denaturation [J].

Bentaleb, A ;

Abele, A ;

Haikel, Y ;

Schaaf, P ;

Voegel, JC .

LANGMUIR, 1998, 14 (22) :6493-6500

[4] Molecular orientation distributions in protein films:: III.: Yeast cytochrome c immobilized on pyridyl disulfide-capped phospholipid bilayers [J].

Edmiston, PL ;

Saavedra, SS .

BIOPHYSICAL JOURNAL, 1998, 74 (02) :999-1006

[5] Molecular orientation distributions in protein films .1. Cytochrome c adsorbed to substrates of variable surface chemistry [J].

Edmiston, PL ;

Lee, JE ;

Cheng, SS ;

Saavedra, SS .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1997, 119 (03) :560-570

[6] Molecular orientation distributions in protein films.: 4.: A multilayer composed of yeast cytochrome c bound through an intermediate streptavidin layer to a planar supported phospholipid bilayer [J].

Edmiston, PL ;

Saavedra, SS .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1998, 120 (08) :1665-1671

[7] UV-visible slab optical waveguide spectroscopy of cytochrome c adsorbed on a liquid-solid interface [J].

Kato, K ;

Takatsu, A ;

Matsuda, N .

CHEMISTRY LETTERS, 1999, (01) :31-32

[8] Molecular orientation in heme protein films adsorbed to hydrophilic and hydrophobic glass surfaces [J].

Lee, JE ;

Saavedra, SS .

LANGMUIR, 1996, 12 (16) :4025-4032

[9] Broad-band attenuated total reflection spectroscopy of a hydrated protein film on a single mode planar waveguide [J].

Mendes, SB ;

Li, LF ;

Burke, JJ ;

Lee, JE ;

Dunphy, DR ;

Saavedra, SS .

LANGMUIR, 1996, 12 (14) :3374-3376

[10] ADSORPTION OF PROTEINS FROM SOLUTION AT THE SOLID-LIQUID INTERFACE [J].

NORDE, W .

ADVANCES IN COLLOID AND INTERFACE SCIENCE, 1986, 25 (04) :267-340