Syntheses of labeled vitamers of folic acid to be used as internal standards in stable isotope dilution assays

被引：44

作者：

Freisleben, A ^{[1
]}

Schieberle, P ^{[1
]}

Rychlik, M ^{[1
]}

机构：

[1] Tech Univ Munich, Inst Lebensmittelchem, D-85748 Garching, Germany

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2002年 / 50卷 / 17期

关键词：

electrospray mass spectrometry; folates; labeled folic acid; stable isotope dilution assay;

D O I：

10.1021/jf025571k

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

[H-2(4)]Folic acid was synthesized by deuterating p-aminobenzoic acid, which was then coupled to glutamic acid and 6-formylpterin. Using [H-2(4)]folic acid as starting component enabled the preparation of labeled vitamers tetrahydrofolate, 5-formyltetrahydrofolate, 5-methyltetrahydrofolate, and 10-formylfolate which were characterized by electrospray mass spectrometry and collision-induced dissociation. The mass spectrometric studies confirmed that the compounds could be used as internal standards in stable isotope dilution assays.

引用

页码：4760 / 4768

页数：9

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