Determination of Macrolide Antibiotics in ChickenTissues by Liquid Chromatography- Electrospray Mass Spectrometry

被引：3
作者：
Salikin, Jamilah [1 ]
Abdullah, Aminah [2 ]
机构：
[1] Minist Hlth, Natl Publ Hlth Lab, Lot 1853 Kampung Melayu, Sungai Buloh 47000, Selangor, Malaysia
[2] Univ Kebangsaan Malaysia, Fac Sci & Technol, Sch Chem Sci & Food Technol, Bangi 43600, Malaysia
来源：
2013 UKM FST POSTGRADUATE COLLOQUIUM | 2013年 / 1571卷
关键词：
Macrolide antibiotics; tylosin; tilmicosin; spiramycin; mass spectrometry; chicken muscle; RESIDUES; EGGS;
D O I：
10.1063/1.4858737
中图分类号：
O59 [应用物理学];
学科分类号：
摘要：
A methodusingliquid chromatography-electrospray mass spectrometry (LC-(ESI)MS) for the simultaneous determination of three macrolides (tylosin, spiramycin and tilmicosin) in poultry muscle has been developed. The drugs were extracted with EDTA McIlvaine buffer, filter through celite 545 and the extracts were cleaned up by SPE Oasis HLB cartridge. Separation was carried out in end-capped silica-based C18 column and mobile phases containing trifluoroacetic acid-acetonitrile with a binary gradient system at a flow rate 0.5 ml/min. Detection was performed by single mass spectrometry with electrospray ionization in the positive mode. Several parameters affecting the mass spectra were studied. Chicken samples from the market were analyzed to check the residue of macrolide antibiotics.
引用
收藏
页码：702 / 709
页数：8
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