The effect of ensiling on PCR-based defection of genetically modified Bt maize

被引：38
作者：
Hupfer, C
Mayer, J
Hotzel, H
Sachse, K
Engel, KH
机构：
[1] Tech Univ Munich, Lehrstuhl Allgemeine Lebensmitteltechnol, D-85350 Freising, Germany
[2] Bayer Landesanstalt Tierzucht, D-85586 Poing Grub, Germany
[3] Bundesinst Gesundheitlichen Verbraucherschultz &, D-07743 Jena, Germany
来源：
EUROPEAN FOOD RESEARCH AND TECHNOLOGY | 1999年 / 209卷 / 05期
关键词：
polymerase chain reaction; genetically modified maize; detection; silage;
D O I：
10.1007/s002170050498
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
The detection of the genetic modification in silage obtained from insect-resistant Bt maize by means of the polymerase chain reaction (PCR) is described. The detectability of the transgene was shown to be dependent on the length of the genomic target sequence chosen for amplication by the PCR. By amplifying a Bt-maize-specific DNA sequence of 211 bp the genetic modification was detected up to 7 months after ensilage. The effect of maize DNA degradation in the course of the ensilage on the detectability of target sequences was demonstrated in model experiments.
引用
收藏
页码：301 / 304
页数：4
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