STABILITY-INDICATING RP-HPLC METHOD FOR SIMULTANEOUS DETERMINATION OF GATIFLOXACIN AND FLURBIPROFEN IN BINARY COMBINATION

A stability-indicating RP-HPLC method is presented for determination of gatifloxacin and flurbiprofen in binary combination. Gatifloxacin, flurbiprofen and their degradation products were detected at 254 nm using a BDS Hypersil C8 (250 X 4.6 mm, 5 mu m) column and mixture of 20 mM phosphate buffer (pH 3.0) and methanol 30:70 v/v as mobile phase. Response was linear over the range of 15-105 mu g mL(-1) for gatifloxacin (r(2) >= 0.998) and of 1.5-10.5 mu g mL(-1) for flurbiprofen (r(2) >= 0.999). The developed method efficiently separated the analytical peaks from degradation products (peak purity index >= 0.9999). The method developed can be applied successfully for determination of gatifloxacin and flurbiprofen in human serum, urine, pharmaceutical formulations, and their stability studies.