Determination of lignans in flaxseed using liquid chromatography with time-of-flight mass spectrometry

被引：51

作者：

Popova, I. E. ^{[1
]}

Hall, C. ^{[2
]}

Kubatova, A. ^{[1
]}

机构：

[1] Univ N Dakota, Dept Chem, Grand Forks, ND 58202 USA

[2] N Dakota State Univ, Dept Cereal & Food Sci, Fargo, ND 58105 USA

来源：

JOURNAL OF CHROMATOGRAPHY A | 2009年 / 1216卷 / 02期

基金：

美国国家科学基金会;

关键词：

Time-of-flight; MS; Flaxseed; Antioxidants; LC/MS; GC/MS; Hydrolysis; Extraction; Lignans; Aglycones; SECOISOLARICIRESINOL DIGLUCOSIDE; PLANT LIGNANS; MAMMALIAN LIGNANS; PHENOLIC-COMPOUNDS; SESAME SEED; HUMAN SERUM; QUANTIFICATION; HPLC; PHYTOESTROGENS; LARICIRESINOL;

D O I：

10.1016/j.chroma.2008.11.063

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new method of flaxseed-derived lignan determination was developed using HPLC with high-resolution time-of-flight MS (TOF-MS), optimized, and compared to two existing methods (HPLC/MS/MS and GC/MS). The limits of detection (LODs) for HPLC/TOF-MS (0.002-0.043 pg) were comparable with those of the optimized and improved HPLC/MS/MS (0.001-0.015 pg), whereas the LODs for the optimized GC/MS were higher (0.02-3.0 pg, yet lower than reported before). Using the newly developed detection and separation methods, several key flaxseed sample preparation parameters (including extraction, hydrolysis, and sample purification) were evaluated resulting in the development of efficient protocol for lignan quantification from flaxseed hulls and embryos. The results confirmed the importance of quantification of both aglycones and unhydrolyzed glucosides in order to obtain the total lignan estimates. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：217 / 229

页数：13

共 60 条

[21] Determination of some phenolic compounds in flax seed and nettle roots by HPLC with coulometric electrode array detection [J].