Evaluation of anti-oxidative, genotoxic and antigenotoxic potency of Codium tomentosum Stackhouse ethanolic extract in human lymphocytes in vitro

被引：33

作者：

Celikler, Serap ^{[1
]}

Vatan, Ozgur ^{[1
]}

Yildiz, Gamze ^{[1
]}

Bilaloglu, Rahmi ^{[1
]}

机构：

[1] Uludag Univ, Sci Arts Fac, Dept Biol, TR-16059 Bursa, Turkey

来源：

FOOD AND CHEMICAL TOXICOLOGY | 2009年 / 47卷 / 04期

关键词：

Codium tomentosum; Oxidative and chromosome damage; Anti-oxidative effect; SCE; MN; CA; ANTIPROLIFERATIVE ACTIVITIES; ANTIBACTERIAL ACTIVITY; MARINE-ALGAE; RED ALGA; DIETARY; SEAWEED; DNA; MICRONUCLEI; INDUCTION; PRODUCTS;

D O I：

10.1016/j.fct.2009.01.010

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

The genome is constantly exposed to agents, both exogenous and endogenous, that damage DNA. Consequently, it is very important that determination of this agents and the protective agents. In this work, we evaluated the antigenotoxic/antimutagenic activity of the crude ethanolic extracts of Codium tomentosurn Stackhouse (Chlorophyceae) (CTE), collected from The Coast of South East Marmara Sea, in human lymphocytes culture in vitro against genotoxic/mutagenic agents MMC, EMS and H2O2 by using chromosome aberration (CA), sister chromatid exchange (SCE) and micronuclei (MN) assays as experimental endpoints. Also, in the present study, we determined total phenolic content and total antioxidant capacity (in soluble lipid and water). In addition, total protein, total carbohydrate, vitamins (A. C and E) and pigments (chlorophyll a, chlorophyll b and carotene) contents were also determined. Results of CA, SCE and MN tests show that CTEs have not shown genotoxic effect. In CTE plus MMC-. EMS- or H2O2- treated cultures, CA, SCE and MN frequency which induced by MMC, EMS or H2O2 has been decreased significantly (p < 0.05-0.001). This is the first report on genotoxicity/antigenotoxicity and anti-oxidative capacity of Codium tomentosum. Our results have clearly shown that CTE has strong anti-oxidative and antigenotoxic effect. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：796 / 801

页数：6

共 54 条

[11] Preliminary study of the in vitro antiproliferative effect of a hydroethanolic extract from the subtropical seaweed Turbinaria ornata (Turner J.!Agardh) on a human non-small-cell bronchopulmonary carcinoma line (NSCLC-N6)
Deslandes, E
Pondaven, P
Auperin, T
Roussakis C, C
Guézennec, J
Stiger, V
Payri, C
[J]. JOURNAL OF APPLIED PHYCOLOGY, 2000, 12 (3-5) : 257 - 262
[12] OXIDATIVE DAMAGE TO DNA IN MAMMALIAN CHROMATIN
DIZDAROGLU, M
[J]. MUTATION RESEARCH, 1992, 275 (3-6): : 331 - 342
[13] Quercetin and myricetin protect against hydrogen peroxide-induced DNA damage (strand breaks and oxidised pyrimidines) in human lymphocytes
Duthie, SJ
Collins, AR
Duthie, GG
Dodson, VL
[J]. MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, 1997, 393 (03) : 223 - 231
[14] Antimicrobial activity of marine organisms collected off the coast of South East India
Ely, R
Supriya, T
Naik, CG
[J]. JOURNAL OF EXPERIMENTAL MARINE BIOLOGY AND ECOLOGY, 2004, 309 (01) : 121 - 127
[15] The in vitro micronucleus technique
Fenech, M
[J]. MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS, 2000, 455 (1-2) : 81 - 95
[16] FUJIMOTO K, 1985, B JPN SOC SCI FISH, V51, P1139
[17] ANTICANCER ACTIVITY OF A NATURAL PRODUCT, VIVA-NATURAL, EXTRACTED FROM UNDARIA-PINNANTIFIDA ON INTRAPERITONEALLY IMPLANTED LEWIS LUNG-CARCINOMA
FURUSAWA, E
FURUSAWA, S
[J]. ONCOLOGY, 1985, 42 (06) : 364 - 369
[18] Phenoloxidase (EC 1.14.18.1) from the byssus gland of Mytilus edulis:: Purification, partial characterization and application for screening products with potential antifouling activities
Hellio, C
Bourgougnon, N
Le Gal, Y
[J]. BIOFOULING, 2000, 16 (2-4) : 235 - 244
[19] Hirasa K.Takemasa., 1998, Spice science and technology
[20] IPCS, 1985, GUID SHORT TERM TEST

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