Stimulated emission depletion microscopy for biological imaging in four dimensions: A review

被引:23
|
作者
Wu, Zhaoyang [1 ]
Xu, Xinzhu [1 ]
Xi, Peng [1 ,2 ]
机构
[1] Peking Univ, Coll Engn, Dept Biomed Engn, Beijing 100871, Peoples R China
[2] Southern Univ Sci & Technol, Coll Engn, Dept Biomed Engn, UTS SUSTech Joint Res Ctr Biomed Mat & Devices, Shenzhen 518055, Guangdong, Peoples R China
基金
中国国家自然科学基金;
关键词
axial resolution; lateral resolution; measurement range; STED; temporal resolution;
D O I
10.1002/jemt.23750
中图分类号
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号
100101 ;
摘要
Stimulated emission depletion (STED) microscopy allows high lateral and axial resolution, long term imaging in living cells. Here we review recent technical advances in STED microscopy, with emphasis on resolution and measurement range of XYZt four dimensions. Different STED technical advances and novel STED probes are discussed with their respective application in biological subcellular imaging. This review may serve as a practical guide for choosing a suitable approach to the advanced STED super-resolution imaging.
引用
收藏
页码:1947 / 1958
页数:12
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