A simple mix-and-read bacteria detection system based on a DNAzyme and a molecular beacon

被引：18

作者：

Cao, Ting ^{[1
,2
,3
,4
]}

Wang, Yongcheng ^{[2
,3
,4
,5
]}

Zhao, Ling-Li ^{[1
]}

Wang, Ye ^{[1
]}

Tao, Ye ^{[2
,3
]}

Heyman, John A. ^{[2
,3
]}

Weitz, David A. ^{[2
,3
,4
]}

Zhou, Ying-Lin ^{[1
]}

Zhang, Xin-Xiang ^{[1
]}

机构：

[1] Peking Univ, Coll Chem, MOE Key Lab Bioorgan Chem & Mol Engn, BNLMS, Beijing 100871, Peoples R China

[2] Harvard Univ, John A Paulson Sch Engn & Appl Sci, Cambridge, MA 02138 USA

[3] Harvard Univ, Dept Phys, Cambridge, MA 02138 USA

[4] Harvard Univ, Wyss Inst Biol Inspired Engn, Boston, MA 02115 USA

[5] Harvard Univ, Dept Chem & Chem Biol, Cambridge, MA 02138 USA

来源：

CHEMICAL COMMUNICATIONS | 2019年 / 55卷 / 51期

基金：

美国国家科学基金会; 中国国家自然科学基金;

关键词：

IN-VITRO SELECTION; BIOSENSOR; PROBES; ASSAY;

D O I：

10.1039/c9cc03746b

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Here, we describe a simple mix-and-read method for the detection of specific bacterial strains that uses a DNAzyme and a molecular beacon to generate a signal. We have greatly improved upon the previously described DNAzyme-based bacteria detection method by eliminating a tedious preparation step while maintaining detection sensitivity.

引用

页码：7358 / 7361

页数：4

共 27 条

[1] A Sensitive DNA Enzyme-Based Fluorescent Assay for Bacterial Detection
Aguirre, Sergio D.
Ali, M. Monsur
Salena, Bruno J.
Li, Yingfu
[J]. BIOMOLECULES, 2013, 3 (03): : 563 - 577
[2] Fluorogenic DNAzyme Probes as Bacterial Indicators
Ali, M. Monsur
Aguirre, Sergio D.
Lazim, Hadeer
Li, Yingfu
[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2011, 50 (16) : 3751 - 3754
[3] POLYMERASE CHAIN REACTION-GENE PROBE DETECTION OF MICROORGANISMS BY USING FILTER-CONCENTRATED SAMPLES
BEJ, AK
MAHBUBANI, MH
DICESARE, JL
ATLAS, RM
[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1991, 57 (12) : 3529 - 3534
[4] Comparison of fluorogenic and chromogenic assay systems in the detection of Escherichia coli O157 by a novel polymyxin-based ELISA
Blais, BW
Leggate, J
Bosley, J
Martinez-Perez, A
[J]. LETTERS IN APPLIED MICROBIOLOGY, 2004, 39 (06) : 516 - 522
[5] Breaker R R, 1994, Chem Biol, V1, P223, DOI 10.1016/1074-5521(94)90014-0
[6] Biochemical Characterization of a Uranyl Ion-Specific DNAzyme
Brown, Andrea K.
Liu, Juewen
He, Ying
Lu, Yi
[J]. CHEMBIOCHEM, 2009, 10 (03) : 486 - 492
[7] Elbaz J, 2010, NAT NANOTECHNOL, V5, P417, DOI [10.1038/nnano.2010.88, 10.1038/NNANO.2010.88]
[8] Gu Q., 2016, PROBIOTICS PREBIOTIC, P135, DOI DOI 10.5772/63117
[9] Highly Specific Recognition of Breast Tumors by an RNA-Cleaving Fluorogenic DNAzyme Probe
He, Shengnan
Qu, Long
Shen, Zhifa
Tan, Ying
Zeng, Meiyun
Liu, Feng
Jiang, Yuyang
Li, Yingfu
[J]. ANALYTICAL CHEMISTRY, 2015, 87 (01) : 569 - 577
[10] An Ultrasensitive Light-up Cu2+ Biosensor Using a New DNAzyme Cleaving a Phosphorothioate-Modified Substrate
Huang, Po-Jung Jimmy
Liu, Juewen
[J]. ANALYTICAL CHEMISTRY, 2016, 88 (06) : 3341 - 3347

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