Direct electrochemiluminescence detection of oxidized DNA in ultrathin films containing [Os(bpy)2(PVP)10]2+

被引：117
作者：
Dennany, L
Forster, RJ
White, B
Smyth, M
Rusling, JF [1 ]
机构：
[1] Dublin City Univ, Sch Chem Sci, NCSR, Dublin 9, Ireland
[2] Univ Connecticut, Dept Chem, Storrs, CT 06269 USA
[3] Univ Connecticut, Ctr Hlth, Dept Pharmacol, Farmington, CT 06032 USA
来源：
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2004年 / 126卷 / 28期
关键词：
D O I：
10.1021/ja048615+
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Direct electrochemiluminescence (ECL) involving oxidized DNA was demonstrated in ultrathin films of cationic polymer [Os(bpy)(2)(PVP)(10)](2+) [PVP = poly(vinyl pyridine)] assembled layer-by-layer with DNA or oligonucleotides. Electrochemically oxidized Os-II sites generated ECL from films containing oxo-guanines on DNA formed by chemical oxidation using Fenton reagent. Films combining DNA, [Ru(bpy)(2)(PVP)(10)](2+), and [Os(bpy)(2)(PVP)(10)](2+) had Os-II sites that produced ECL specific for oxidized DNA, and Ru-II sites gave ECL from reaction with oxo-adenines, chemically damaged DNA, and possibly from cleaved DNA strands.
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收藏
页码：8835 / 8841
页数：7
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