Production and characterization of a polyclonal antibody for Os(II) and Ru(II) polypyridyl complexes

被引：3

作者：

O'Connor, Maire B.

Killard, Anthony J. ^{[1
]}

O'Kennedy, Richard

Forster, Robert J.

Smyth, Malcolm R.

机构：

[1] Dublin City Univ, Natl Ctr Sensor Res, Sch Chem Sci, Dublin 9, Ireland

[2] Dublin City Univ, Natl Ctr Sensor Res, Sch Biotechnol, Dublin 9, Ireland

来源：

JOURNAL OF INORGANIC BIOCHEMISTRY | 2006年 / 100卷 / 07期

关键词：

antibody; osmium(II); ruthenium(II); polypyridyl;

D O I：

10.1016/j.jinorgbio.2006.02.002

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The characterization of a polyclonal antibody produced via immunization with an [Os(bpy)(2)dcbpy] hapten is described. Bpy is 2,2'-bipyridine and dcbpy is 2,2'-bipyridine-4,4'-dicarboxylic acid. The cross-reactivity of the antibody for the Ru(II) analogue of the hapten was also investigated. Large increases in the emission and luminescent lifetime of a series of Os and Ru complexes were observed on binding of the antibody. Association equilibrium constants were derived from luminescence titration data and were found to be 5.6 x 10(8) and 5.0 x 10(8) M-1 for [Os(bpy)(2)dcbpy] and [Ru(bpy)(2)dcbpy], respectively. Spectroscopic changes were likely due to the exclusion of H2O from the complex/antibody binding cleft and blocking of vibrational relaxation pathways of the Os/Ru excited state. D2O/H2O experiments confirmed that the antibody protected approx. 82% of [Os(bpy)(2)dcbpy] and 80% of [Ru(bpy)(2)dcbpy] from excited state deactivation by the aqueous solvent. (c) 2006 Elsevier Inc. All rights reserved.

引用

页码：1252 / 1259

页数：8

共 32 条

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