Genetic and biological characterization of Muko virus, a new distinct member of the species Great Island virus (genus Orbivirus, family Reoviridae), isolated from ixodid ticks in Japan

被引：17

作者：

Ejiri, Hiroko ^{[1
]}

Lim, Chang-Kweng ^{[2
]}

Isawa, Haruhiko ^{[1
]}

Kuwata, Ryusei ^{[3
]}

Kobayashi, Daisuke ^{[1
]}

Yamaguchi, Yukie ^{[2
]}

Takayama-Ito, Mutsuyo ^{[2
]}

Kinoshita, Hitomi ^{[2
]}

Kakiuchi, Satsuki ^{[2
]}

Horiya, Madoka ^{[2
]}

Kotaki, Akira ^{[2
]}

Takasaki, Tomohiko ^{[2
]}

Maeda, Ken ^{[3
]}

Hayashi, Toshihiko ^{[1
]}

Sasaki, Toshinori ^{[1
]}

Kobayashi, Mutsuo ^{[1
]}

Saijo, Masayuki ^{[2
]}

Sawabe, Kyoko ^{[1
]}

机构：

[1] Natl Inst Infect Dis, Dept Med Entomol, Shinjuku Ku, Tokyo 1628640, Japan

[2] Natl Inst Infect Dis, Dept Virol 1, Shinjuiku Ku, Tokyo 1628640, Japan

[3] Yamaguchi Univ, Fac Agr, Lab Vet Microbiol, Yamaguchi 7538515, Japan

来源：

ARCHIVES OF VIROLOGY | 2015年 / 160卷 / 12期

基金：

日本学术振兴会;

关键词：

KEMEROVO SEROGROUP VIRUSES; RNA BLOT HYBRIDIZATION; REASSORTMENT INVITRO; BLUETONGUE VIRUS; TRIBEC VIRUS; COMPLETE SEQUENCE; MIGRATORY BIRDS; SEABIRD HOST; SEROCOMPLEX; RELATEDNESS;

D O I：

10.1007/s00705-015-2588-7

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Among the tick-borne orbiviruses (genus Orbivirus, family Reoviridae), 36 serotypes are currently classified within a single virus species, Great Island virus. In this study, we report the first characterization of a tick-borne orbivirus isolated from the tick Ixodes turdus in Japan, which we identified as a new member of the species Great Island virus. The virus isolate, designated Muko virus (MUV), replicated and induced cytopathic effects in BHK-21, Vero E6, and CCL-141 cells and caused high mortality in suckling mice after intracerebral inoculation. Full genome sequence analysis showed that MUV shared the greatest phylogenetic similarity with Tribec. virus in terms of the amino acid sequences of all viral proteins except for outer capsid protein 1 (OC1; VP4 of MUV). Analysis of genome segment 9 in MUV detected an uninterrupted open reading frame that overlaps with VP6 (Hel), which putatively encodes a molecular and functional equivalent of NS4 from Great Island virus. Our study provides new insights into the geographic distribution, genetic diversity, and evolutionary history of the members of the species Great Island virus.

引用

页码：2965 / 2977

页数：13

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