Calcium mobilizing treatment acts as a co-signal for TLR-mediated induction of Interleukin-12 (IL-12p70) secretion by murine bone marrow-derived dendritic cells

被引:7
|
作者
Huang, Emily [1 ]
Showalter, Loral [1 ]
Xu, Shuwen [1 ]
Czernliecki, Brian J. [1 ]
Koski, Gary K. [1 ]
机构
[1] Kent State Univ, Dept Biol Sci, Sch Biomed Sci, Kent, OH 44242 USA
关键词
Dentritic cell; IL-12; Toll-like receptor; Calcium mobilization; Th polarization;
D O I
10.1016/j.cellimm.2017.01.010
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
We sought to determine whether pharmacological calcium-mobilizing agents could act in cooperation with Toll-like receptor (TLR) signals to induce high-level IL-12 production from murine bone marrow derived dendritic cells. We found that calcium mobilization alone induced no IL-12, yet dramatically enhanced IL-12p70 secretion elicited by TLR ligands. Enhanced IL-12 production induced by calcium ionophore plus single TLR ligands, but not through dual TLR ligands, was inhibited by the calcineurin antagonist cyclosporine A, suggesting divergent mechanisms of IL-12 induction. Dendritic cells activated with calciumionophore plus the TLR9 ligand ODN1826 could induce Th1 polarization in nave murine CD4(POS) T cells at levels equal or superior to dendritic cells activated with the most efficient TLR ligand pairing; ODN1826 plus bacterial lipopolysaccharide. Parallel analysis of 38 inflammation-associated soluble products showed calciumionophore enhancement was restricted to a small set of factors. These data demonstrate previously undocumented activation co-signals for IL-12 production by dendritic cells. (C) 2017 Elsevier Inc. All rights reserved.
引用
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页码:26 / 35
页数:10
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