Calcium mobilizing treatment acts as a co-signal for TLR-mediated induction of Interleukin-12 (IL-12p70) secretion by murine bone marrow-derived dendritic cells

被引：7

作者：

Huang, Emily ^{[1
]}

Showalter, Loral ^{[1
]}

Xu, Shuwen ^{[1
]}

Czernliecki, Brian J. ^{[1
]}

Koski, Gary K. ^{[1
]}

机构：

[1] Kent State Univ, Dept Biol Sci, Sch Biomed Sci, Kent, OH 44242 USA

来源：

CELLULAR IMMUNOLOGY | 2017年 / 314卷

关键词：

Dentritic cell; IL-12; Toll-like receptor; Calcium mobilization; Th polarization;

D O I：

10.1016/j.cellimm.2017.01.010

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We sought to determine whether pharmacological calcium-mobilizing agents could act in cooperation with Toll-like receptor (TLR) signals to induce high-level IL-12 production from murine bone marrow derived dendritic cells. We found that calcium mobilization alone induced no IL-12, yet dramatically enhanced IL-12p70 secretion elicited by TLR ligands. Enhanced IL-12 production induced by calcium ionophore plus single TLR ligands, but not through dual TLR ligands, was inhibited by the calcineurin antagonist cyclosporine A, suggesting divergent mechanisms of IL-12 induction. Dendritic cells activated with calciumionophore plus the TLR9 ligand ODN1826 could induce Th1 polarization in nave murine CD4(POS) T cells at levels equal or superior to dendritic cells activated with the most efficient TLR ligand pairing; ODN1826 plus bacterial lipopolysaccharide. Parallel analysis of 38 inflammation-associated soluble products showed calciumionophore enhancement was restricted to a small set of factors. These data demonstrate previously undocumented activation co-signals for IL-12 production by dendritic cells. (C) 2017 Elsevier Inc. All rights reserved.

引用

页码：26 / 35

页数：10