Urinary cell-free DNA is a versatile analyte for monitoring infections of the urinary tract

被引：123

作者：

Burnham, Philip ^{[1
]}

Dadhania, Darshana ^{[2
,3
]}

Heyang, Michael ^{[1
]}

Chen, Fanny ^{[1
]}

Westblade, Lars F. ^{[4
,5
]}

Suthanthiran, Manikkam ^{[2
,3
]}

Lee, John Richard ^{[2
,3
]}

De Vlaminck, Iwijn ^{[1
]}

机构：

[1] Cornell Univ, Meinig Sch Biomed Engn, Ithaca, NY 14853 USA

[2] Weill Cornell Med, Div Nephrol & Hypertens, Dept Med, New York, NY 10065 USA

[3] New York Presbyterian Hosp, Dept Transplantat Med, Weill Cornell Med Ctr, New York, NY 10065 USA

[4] Weill Cornell Med, Dept Pathol & Lab Med, New York, NY 10065 USA

[5] Weill Cornell Med, Div Infect Dis, Dept Med, New York, NY 10065 USA

来源：

NATURE COMMUNICATIONS | 2018年 / 9卷

关键词：

RENAL-TRANSPLANTATION; NONINVASIVE DIAGNOSIS; LIBRARY PREPARATION; MITOCHONDRIAL-DNA; READ ALIGNMENT; BK VIRUS; DONOR; RECIPIENTS; SEQUENCE; PLASMA;

D O I：

10.1038/s41467-018-04745-0

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Urinary tract infections are one of the most common infections in humans. Here we tested the utility of urinary cell-free DNA (cfDNA) to comprehensively monitor host and pathogen dynamics in bacterial and viral urinary tract infections. We isolated cfDNA from 141 urine samples from a cohort of 82 kidney transplant recipients and performed next-generation sequencing. We found that urinary cfDNA is highly informative about bacterial and viral composition of the microbiome, antimicrobial susceptibility, bacterial growth dynamics, kidney allograft injury, and host response to infection. These different layers of information are accessible from a single assay and individually agree with corresponding clinical tests based on quantitative PCR, conventional bacterial culture, and urinalysis. In addition, cfDNA reveals the frequent occurrence of pathologies that remain undiagnosed with conventional diagnostic protocols. Our work identifies urinary cfDNA as a highly versatile analyte to monitor infections of the urinary tract.

引用

页数：10

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