Molecular typing of Treponema pallidum isolates from Buenos Aires, Argentina: Frequent Nichols-like isolates and low levels of macrolide resistance

被引：27

作者：

Gallo Vaulet, Lucia ^{[1
,2
]}

Grillova, Linda ^{[3
]}

Mikalova, Lenka ^{[3
]}

Casco, Ricardo ^{[4
]}

Rodriguez Fermepin, Marcelo ^{[1
,2
]}

Pando, Maria A. ^{[5
]}

Smajs, David ^{[3
]}

机构：

[1] Univ Buenos Aires, Fac Farm & Bioquim, Dept Bioquim Clin, Catedra Microbiol Clin, Buenos Aires, DF, Argentina

[2] Univ Buenos Aires, Inst Fisiopatol & Bioquim Clin INFIBIOC, Buenos Aires, DF, Argentina

[3] Masaryk Univ, Dept Biol, Fac Med, Brno, Czech Republic

[4] Univ Buenos Aires, Hosp Clin Jose de San Martin, PETS, Buenos Aires, DF, Argentina

[5] Univ Buenos Aires, CONICET, Inst Invest Biomed Retrovirus & SIDA INBIRS, Buenos Aires, DF, Argentina

来源：

PLOS ONE | 2017年 / 12卷 / 02期

关键词：

SYPHILIS-CAUSING STRAINS; RIBOSOMAL-RNA A2058G; AZITHROMYCIN RESISTANCE; SAN-FRANCISCO; UNITED-STATES; TP0548; GENE; PREVALENCE; EPIDEMIOLOGY; MUTATIONS; SEQUENCE;

D O I：

10.1371/journal.pone.0172905

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A total of 54 clinical samples, including genital lesion swabs, whole blood and cerebrospinal fluid from patients diagnosed with syphilis were collected in 2006 and in 2013 in Buenos Aires, Argentina. Treponemal DNA was detected in 43 of the analyzed samples (79.6%) and further analyzed using Sequencing-based molecular typing (SBMT) and Enhanced CDC-typing (ECDCT). By SBMT, 10 different Treponema pallidum subsp. pallidum (TPA) genotypes were found, of which six were related to the TPA SS14 strain, and four to the TPA Nichols strain. The 23S rRNA gene was amplified in samples isolated from 42 patients, and in six of them (14.3%), either the A2058G (four patients, 9.5%) or the A2059G (two patients, 4.8%) mutations were found. In addition to Taiwan, Madagascar and Peru, Argentina is another country where the prevalence of Nichols-like isolates (26.8%) is greater than 10%.

引用

页数：14

共 55 条

[1] Treponema pallidum pallidum Genotypes and Macrolide Resistance Status in Syphilitic Lesions among Patients at 2 Sexually Transmitted Infection Clinics in Lima, Peru [J].

Antonio Flores, Juan ;

Keith Vargas, Silver ;

Ramos Leon, Segundo ;

Giancarlo Perez, Danny ;

Beatriz Ramos, Lourdes ;

Chow, Jeremy ;

Konda, Kelika Anne ;

Mauricio Calvo, Gino ;

Salvatierra, Hector J. ;

Klaussner, Jeffrey D. ;

Fernando Caceres, Carlos .

SEXUALLY TRANSMITTED DISEASES, 2016, 43 (07) :465-466

[2] Median-joining networks for inferring intraspecific phylogenies [J].

Bandelt, HJ ;

Forster, P ;

Röhl, A .

MOLECULAR BIOLOGY AND EVOLUTION, 1999, 16 (01) :37-48

[3] High prevalence of azithromycin resistance to Treponema pallidum in geographically different areas in China [J].

Chen, X. -S. ;

Yin, Y. -P. ;

Wei, W. -H. ;

Wang, H. -C. ;

Peng, R. -R. ;

Zheng, H. -P. ;

Zhang, J. -P. ;

Zhu, B. -Y. ;

Liu, Q. -Z. ;

Huang, S. -J. .

CLINICAL MICROBIOLOGY AND INFECTION, 2013, 19 (10) :975-979

[4] Secondary Syphilis in Cali, Colombia: New Concepts in Disease Pathogenesis [J].

Cruz, Adriana R. ;

Pillay, Allan ;

Zuluaga, Ana V. ;

Ramirez, Lady G. ;

Duque, Jorge E. ;

Aristizabal, Gloria E. ;

Fiel-Gan, Mary D. ;

Jaramillo, Roberto ;

Trujillo, Rodolfo ;

Valencia, Carlos ;

Jagodzinski, Linda ;

Cox, David L. ;

Radolf, Justin D. ;

Salazar, Juan C. .

PLOS NEGLECTED TROPICAL DISEASES, 2010, 4 (05)

[5] Molecular Typing of Treponema pallidum: a 5-Year Surveillance in Shanghai, China [J].

Dai, Ting ;

Li, Kang ;

Lu, Haikong ;

Gu, Xin ;

Wang, Qianqiu ;

Zhou, Pingyu .

JOURNAL OF CLINICAL MICROBIOLOGY, 2012, 50 (11) :3674-3677

[6] First report on sexually transmitted infections among trans (male to female transvestites, transsexuals, or transgender) and male sex workers in Argentina: high HIV, HPV, HBV, and syphilis prevalence [J].

dos Ramos Farias, Maria Sol ;

Noe Garcia, Maria ;

Reynaga, Elena ;

Romero, Marcela ;

Gallo Vaulet, Maria Lucia ;

Rodriguez Fermepin, Marcelo ;

Fernandez Toscano, Mauro ;

Rey, Jorge ;

Marone, Ruben ;

Squiquera, Luis ;

Gonzalez, Joaquin V. ;

Basiletti, Jorge ;

Alejandra Picconi, Maria ;

Pando, Maria A. ;

Mercedes Avila, Maria .

INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES, 2011, 15 (09) :E635-E640

[7]

Falistocco C, 2012, VIH SIFILIS SEROPREV

[8]

Flasarova M., 2006, Epidemiologie Mikrobiologie Imunologie, V55, P105

[9] Sequencing-based Molecular Typing of Treponema pallidum Strains in the Czech Republic: All Identified Genotypes are Related to the Sequence of the SS14 Strain [J].

Flasarova, Magdalena ;

Pospisilova, Petra ;

Mikalova, Lenka ;

Valisova, Zuzana ;

Dastychova, Eliska ;

Strnadel, Radim ;

Kuklova, Ivana ;

Woznicova, Vladana ;

Zakoucka, Hana ;

Smajs, David .

ACTA DERMATO-VENEREOLOGICA, 2012, 92 (06) :669-674

[10] Molecular Typing of Treponema pallidum Clinical Strains from Lisbon, Portugal [J].

Florindo, C. ;

Reigado, V. ;

Gomes, J. P. ;

Azevedo, J. ;

Santo, I. ;

Borrego, M. J. .

JOURNAL OF CLINICAL MICROBIOLOGY, 2008, 46 (11) :3802-3803

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