A cytoskeleton-associated protein, TMAP/CKAP2, is involved in the proliferation of human foreskin fibroblasts

被引:27
作者
Jeon, Sang-Min
Choi, Bongkun
Hong, Kyung Uk
Kim, Eunhee
Seong, Yeon-Sun
Bae, Chang-Dae
Park, Joobae [1 ]
机构
[1] Sungkyunkwan Univ, Sch Med, Dept Mol Cell Biol, Suwon, South Korea
[2] Sungkyunkwan Univ, Sch Med, Samsung Biomed Res Inst, Suwon, South Korea
[3] Dankook Univ, Coll Med, Dept Biochem, Chunan, South Korea
关键词
TMAP; CKAP2; G1; cell cycle; cytoskeleton; proliferation;
D O I
10.1016/j.bbrc.2006.07.046
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Previously, we reported the cloning of a cytoskeleton-associated protein, TMAP/CKAP2, which was up-regulated in primary human gastric cancers. Although TMAP/CKAP2 has been found to be expressed in most cancer cell lines examined, the function of CKAP2 is not known. In this study, we found that TMAP/CKAP2 was not expressed in G0/G1 arrested HFFs, but that it was expressed in actively dividing cells. After initiating the cell cycle, TMAP/CKAP2 levels remained low throughout most of the G1 phase, but gradually increased between late G1 and G2/M. Knockdown of TMAP/CKAP2 reduced pRB phosphorylation and increased p27 expression, and consequently reduced HFF proliferation, whereas constitutive TMAP/CKAP2 expression increased pR13 phosphorylation and enhanced proliferation. Our results show that this novel cytoskeleton-associated protein is expressed cell cycle dependently and that it is involved in cell proliferation. (c) 2006 Elsevier Inc. All rights reserved.
引用
收藏
页码:222 / 228
页数:7
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