Determination of aromatic amino acid content in cooked meat by derivative spectrophotometry: Implications for nutritional quality of meat

The technique of second derivative spectro photometry was used to determine the level and the heat stability of the three aromatic amino acids (tryptophan, tyrosine and phenylalanine) in bovine meat (M. Longissimus thoraci). This paper presents a method which measures the second derivative absorbance values at a wavelength specifically assigned to each aromatic amino acid with corrections for the interference from other amino acids at the same wavelength. Three cooking temperatures were tested in this study (60. 100 and 140 degrees C. Due to important cooking losses, results differ slightly according to the method of calculation (level expressed by gram of wet meat or by gram of proteins). Whatever the calculation method, heating at 60 degrees C had little effect on aromatic acid levels while higher temperatures had a dramatic effect on aromatic amino acids stability. The stability of the three aromatic amino acids during cooking decreased in the order tryptophan > phenylalanine > tyrosine. (C) 2008 Elsevier Ltd. All rights reserved.