Combined use of the BinaxNOW Staphylococcus aureus test with the Clearview PBP2a assay for the early detection of methicillin-resistant S. aureus from positive blood cultures

被引:10
作者
Kong, Haishen [1 ]
Tong, Ling [2 ]
Zhang, Weili [1 ]
Fu, Yajie [2 ]
Li, Xuefen [1 ,2 ]
机构
[1] Zhejiang Univ, Affiliated Hosp 1, Sch Med, State Key Lab Diag & Treatment Infect Dis, Hangzhou, Zhejiang, Peoples R China
[2] Zhejiang Univ, Sch Med, Affiliated Hosp 1, Ctr Clin Lab, Hangzhou 310003, Zhejiang, Peoples R China
关键词
Methicillin-resistant Staphylococcus aureus; Bacteremia; Identification; Immunochromatographic assay; Blood culture; RAPID IDENTIFICATION; BACTEREMIA; LENGTH; HOSPITALIZATION; PATIENT; COST; MECC;
D O I
10.1016/j.diagmicrobio.2013.11.018
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
The use of combination 2 novel immunochromatographic assays for same-day detection of methicillinresistant Staphylococcus aureus (MRSA) from positive blood cultures was evaluated. Compared to the standard culture methods, the BinaxNOW (R) S.aureus test demonstrated 98.7% sensitivity and 100% specificity in correctly identifying S. aureus. The sensitivity and specificity of the Clearview (R) PBP2a assay in differentiating MRSA from methicillin-susceptible S. aureus were 97.1% and 100%. (C) 2014 Elsevier Inc. All rights reserved.
引用
收藏
页码:226 / 228
页数:3
相关论文
共 17 条
[1]  
[Anonymous], 2012, CLSI version M100-S22, V32, P1
[2]   Evaluation of the Nanosphere Verigene Gram-Positive Blood Culture Assay with the VersaTREK Blood Culture System and Assessment of Possible Impact on Selected Patients [J].
Beal, Stacy G. ;
Ciurca, Jane ;
Smith, Geremy ;
John, Jeffrey ;
Lee, Francesca ;
Doern, Christopher D. ;
Gander, Rita M. .
JOURNAL OF CLINICAL MICROBIOLOGY, 2013, 51 (12) :3988-3992
[3]   Effects of rapid detection of bloodstream infections on length of hospitalization and hospital charges [J].
Beekmann, SE ;
Diekema, DJ ;
Chapin, KC ;
Doern, GV .
JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (07) :3119-3125
[4]   Controlled Multicenter Evaluation of a Bacteriophage-Based Method for Rapid Detection of Staphylococcus aureus in Positive Blood Cultures [J].
Bhowmick, T. ;
Mirrett, S. ;
Reller, L. B. ;
Price, C. ;
Qi, C. ;
Weinstein, M. P. ;
Kirn, T. J. .
JOURNAL OF CLINICAL MICROBIOLOGY, 2013, 51 (04) :1226-1230
[5]   High Proportion of Wrongly Identified Methicillin-Resistant Staphylococcus aureus Carriers by Use of a Rapid Commercial PCR Assay Due to Presence of Staphylococcal Cassette Chromosome Element Lacking the mecA Gene [J].
Blanc, Dominique S. ;
Basset, Patrick ;
Nahimana-Tessemo, Immaculee ;
Jaton, Katia ;
Greub, Gilbert ;
Zanetti, Giorgio .
JOURNAL OF CLINICAL MICROBIOLOGY, 2011, 49 (02) :722-724
[6]   Use of Vitek 2 Antimicrobial Susceptibility Profile To Identify mecC in Methicillin-Resistant Staphylococcus aureus [J].
Cartwright, Edward J. P. ;
Paterson, Gavin K. ;
Raven, Kathy E. ;
Harrison, Ewan M. ;
Gouliouris, Theodore ;
Kearns, Angela ;
Pichon, Bruno ;
Edwards, Giles ;
Skov, Robert L. ;
Larsen, Anders R. ;
Holmes, Mark A. ;
Parkhill, Julian ;
Peacock, Sharon J. ;
Toeroek, M. Estee .
JOURNAL OF CLINICAL MICROBIOLOGY, 2013, 51 (08) :2732-2734
[7]   Epidemiological and Genetic Diversity of Staphylococcus aureus Causing Bloodstream Infection in Shanghai, 2009-2011 [J].
Chen, Xu ;
Wang, Wen-Kui ;
Han, Li-Zhong ;
Liu, Ying ;
Zhang, Hong ;
Tang, Jin ;
Liu, Qing-Zhong ;
Huangfu, Yu-Chan ;
Ni, Yu-Xing .
PLOS ONE, 2013, 8 (09)
[8]   The impact of methicillin-resistance in Staphylococcus aureus bacteremia on patient outcomes:: Mortality, length of stay, and hospital charges [J].
Cosgrove, SE ;
Qi, YL ;
Kaye, KS ;
Harbarth, S ;
Karchmer, AW ;
Carmeli, Y .
INFECTION CONTROL AND HOSPITAL EPIDEMIOLOGY, 2005, 26 (02) :166-174
[9]   Evaluation of the BinaxNOW Staphylococcus aureus Test for Rapid Identification of Gram-Positive Cocci from VersaTREK Blood Culture Bottles [J].
Dhiman, Neelam ;
Trienski, Tamara L. ;
DiPersio, Linda P. ;
DiPersio, Joseph R. .
JOURNAL OF CLINICAL MICROBIOLOGY, 2013, 51 (09) :2939-2942
[10]  
Ly Tam, 2008, Ther Clin Risk Manag, V4, P637