Effects of isolation-rearing on serotonin-1A and M1-muscarinic receptor messenger RNA expression in the hipocampal formation of rats

被引：13

作者：

Del-Bel, EA

Joca, SRL

Padovan, CM

Guimaraes, FS

机构：

[1] Sch Med, Dept Pharmacol, BR-14049900 Ribeirao Preto, SP, Brazil

[2] Sch Odontol, Dept MEF Physiol, BR-14040904 Ribeirao Preto, SP, Brazil

来源：

NEUROSCIENCE LETTERS | 2002年 / 332卷 / 02期

基金：

巴西圣保罗研究基金会;

关键词：

serotonin; in situ hybridization; depression; aggression; hippocampus;

D O I：

10.1016/S0304-3940(02)00933-3

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The aim of the present work was to investigate if isolation rearing could change 5-HT1A or M1 muscarinic receptors messenger RNA (mRNA) expression in the hippocampal formation. Male Wistar rats were isolated either in single cages or in groups of six per cage soon after wearing during 30 days. After this period they were sacrificed and their brains removed for 'in situ' hybridization study using P-32-labeled oligonucleotide probes complementary to 5-HT1A or M1 muscarinic receptor mRNA. The results were analyzed by computerized densitometry. They showed a significant (P < 0.05, Mann-Whitney test) serotonin 1A (5-HT1A) mRNA expression increase in the dentate gyrus and CA3 areas of isolated animals. The signal also tended to be higher (P < 0.10) in CA1 and CA4 regions. No significant change on M1 mRNA expression was found. These results may reflect up-regulation of 5-HT1A gene transcription in response to deficits in hippocampal serotonin neurotransmission induced by social isolation. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：123 / 126

页数：4

共 21 条

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