Efficient and precise editing of endogenous transcripts with SNAP-tagged ADARs

被引：112
作者：
Vogel, Paul [1 ]
Moschref, Matin [1 ]
Li, Qin [2 ]
Merkle, Tobias [1 ]
Selvasaravanan, Karthika D. [1 ]
Li, Jin Billy [2 ]
Stafforst, Thorsten [1 ]
机构：
[1] Univ Tubingen, Interfac Inst Biochem, Tubingen, Germany
[2] Stanford Univ, Dept Genet, Stanford, CA 94305 USA
来源：
NATURE METHODS | 2018年 / 15卷 / 07期
基金：
美国国家卫生研究院; 欧洲研究理事会;
关键词：
SEQUENCING DATA; RNA; MUTATIONS; SPECIFICITY; ALIGNMENT; PROTEINS; ENZYME; SITES; TOOL;
D O I：
10.1038/s41592-018-0017-z
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Molecular tools that target RNA at specific sites allow recoding of RNA information and processing. SNAP-tagged deaminases guided by a chemically stabilized guide RNA can edit targeted adenosine to inosine in several endogenous transcripts simultaneously, with high efficiency (up to 90%), high potency, sufficient editing duration, and high precision. We used adenosine deaminases acting on RNA (ADARs) fused to SNAP-tag for the efficient and concurrent editing of two disease-relevant signaling transcripts, KRAS and STAT1. We also demonstrate improved performance compared with that of the recently described Cas13b-ADAR.
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页码：535 / +
页数：6
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