Mannose-binding lectin is present in human semen and modulates cellular adhesion of Neisseria gonorrhoeae in vitro

被引：4

作者：

Wing, J. B. ^{[1
]}

Jack, D. L. ^{[1
]}

Lee, M. E. ^{[1
]}

Pacey, A. A. ^{[2
]}

Kinghorn, G. R. ^{[3
]}

Read, R. C. ^{[1
]}

机构：

[1] Univ Sheffield, Sch Med, Acad Unit Infect & Immun, Sheffield S10 2RX, S Yorkshire, England

[2] Univ Sheffield, Sch Med, Acad Unit Reprod & Dev Med, Sheffield S10 2RX, S Yorkshire, England

[3] Royal Hallamshire Hosp, Dept Genitourinary Med, Sheffield S10 2JF, S Yorkshire, England

来源：

CLINICAL AND EXPERIMENTAL IMMUNOLOGY | 2009年 / 157卷 / 03期

关键词：

complement; infections; mucosal immunity; reproductive immunology; URETHRAL EPITHELIAL-CELLS; RECOGNITION MOLECULES; BACTERICIDAL ACTIVITY; PROTEIN; MENINGITIDIS; INFECTION; COMPLEMENT; MBL; GENE; MACROPHAGES;

D O I：

10.1111/j.1365-2249.2009.03984.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Mannose-binding lectin (MBL) is an innate immune molecule present in blood and some mucosal tissues, which can influence microbial attachment and inflammatory responses of host cells during infection. In this study MBL was found to be present at a low concentration in semen samples in the range 1.2-24.9 ng/ml. Co-incubation of bacteria with semen resulted in the binding of MBL to the bacterial surface. Neisseria gonorrhoeae is a common cause of genitourinary infection. MBL bound to N. gonorrhoeae with strain-to-strain variation in the intensity of binding and nature of the bacterial receptor. Pretreatment with MBL concentrations similar to those found in human serum modulated the adhesion of N. gonorrhoeae strain FA1090 but not strain MS11 to epithelial cells. This effect was dose-dependent. This work demonstrates that MBL is present in human semen and modifies cellular responses to N. gonorrhoeae in a concentration-dependent manner.

引用

页码：408 / 414

页数：7

共 38 条

[1] Relation between recurrent vulvovaginal candidiasis, vaginal concentrations of mannose-binding lectin, and a mannose-binding lectin gene polymorphism in latvian women
Babula, O
Lazdane, G
Kroica, J
Ledger, WJ
Witkin, SS
[J]. CLINICAL INFECTIOUS DISEASES, 2003, 37 (05) : 733 - 737
[2] DANKERT J, 2003, INFECT DIS, P2173
[3] The lipopolysaccharide structures of Salmonella enterica serovar Typhimurium and Neisseria gonorrhoeae determine the attachment of human mannose-binding lectin to intact organisms
Devyatyrova-Johnson, M
Rees, IH
Robertson, BD
Turner, MW
Klein, NJ
Jack, DL
[J]. INFECTION AND IMMUNITY, 2000, 68 (07) : 3894 - 3899
[4] Mannose-binding lectin binds to two major outer membrane proteins, opacity protein and porin, of Neisseria meningitidis
Estabrook, MM
Jack, DL
Klein, NJ
Jarvis, GA
[J]. JOURNAL OF IMMUNOLOGY, 2004, 172 (06) : 3784 - 3792
[5] Sialylation of Neisseria meningitidis lipooligosaccharide inhibits serum bactericidal activity by masking lacto-N-neotetraose
Estabrook, MM
Griffiss, JM
Jarvis, GA
[J]. INFECTION AND IMMUNITY, 1997, 65 (11) : 4436 - 4444
[6] A HUMAN-SERUM MANNOSE-BINDING PROTEIN INHIBITS INVITRO INFECTION BY THE HUMAN IMMUNODEFICIENCY VIRUS
EZEKOWITZ, RAB
KUHLMAN, M
GROOPMAN, JE
BYRN, RA
[J]. JOURNAL OF EXPERIMENTAL MEDICINE, 1989, 169 (01) : 185 - 196
[7] C1q and MBL, components of the innate immune system, influence monocyte cytokine expression
Fraser, Deborah A.
Bohlson, Suzanne S.
Jasinskiene, Nijole
Rawal, Nenoo
Palmarini, Gail
Ruiz, Sol
Rochford, Rosemary
Tenner, Andrea J.
[J]. JOURNAL OF LEUKOCYTE BIOLOGY, 2006, 80 (01) : 107 - 116
[8] Regulation of the mannan-binding lectin pathway of complement on Neisseria gonorrhoeae by C1-inhibitor and α2-macroglobulin
Gulati, S
Sastry, K
Jensenius, JC
Rice, PA
Ram, S
[J]. JOURNAL OF IMMUNOLOGY, 2002, 168 (08) : 4078 - 4086
[9] Immortalization of human urethral epithelial cells:: A model for the study of the pathogenesis of and the inflammatory cytokine response to Neisseria gonorrhoeae infection
Harvey, HA
Post, DMB
Apicella, MA
[J]. INFECTION AND IMMUNITY, 2002, 70 (10) : 5808 - 5815
[10] Receptor-mediated endocytosis of Neisseria gonorrhoeae into primary human urethral epithelial cells:: the role of the asialoglycoprotein receptor
Harvey, HA
Jennings, MP
Campbell, CA
Williams, R
Apicella, MA
[J]. MOLECULAR MICROBIOLOGY, 2001, 42 (03) : 659 - 672

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