Isolation and characterization of histone H3 lysine 4 demethylase-containing complexes

被引：9
作者：
Lee, Min Gyu [1 ]
Wynder, Christopher [1 ]
Norman, Jessica [1 ]
Shiekhattar, Ramin [1 ]
机构：
[1] Wistar Inst Anat & Biol, Philadelphia, PA 19104 USA
来源：
METHODS | 2006年 / 40卷 / 04期
关键词：
histone methylation; histone demethylase; protein complex; BHC110/LSD1;
D O I：
10.1016/j.ymeth.2006.07.030
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Histone methylation is involved in the regulation of many cellular processes. In the past 2 years, several histone demethylases including BHC110/LSD1 have been characterized. BHC110, the first known histone lysine demethylase, removes methyl groups from methylated histone H3 lysine 4 and has been found in many multi-protein complexes. Using one-step affinity purification, we have isolated enzymatically active BHC110-containing complexes. Here, we detail the methods used for the isolation and characterization of these histone demethylase complexes from a human stable cell line. (c) 2006 Elsevier Inc. All rights reserved.
引用
收藏
页码：327 / 330
页数：4
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