Isolation and characterization of histone H3 lysine 4 demethylase-containing complexes

被引：9

作者：

Lee, Min Gyu ^{[1
]}

Wynder, Christopher ^{[1
]}

Norman, Jessica ^{[1
]}

Shiekhattar, Ramin ^{[1
]}

机构：

[1] Wistar Inst Anat & Biol, Philadelphia, PA 19104 USA

来源：

METHODS | 2006年 / 40卷 / 04期

关键词：

histone methylation; histone demethylase; protein complex; BHC110/LSD1;

D O I：

10.1016/j.ymeth.2006.07.030

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Histone methylation is involved in the regulation of many cellular processes. In the past 2 years, several histone demethylases including BHC110/LSD1 have been characterized. BHC110, the first known histone lysine demethylase, removes methyl groups from methylated histone H3 lysine 4 and has been found in many multi-protein complexes. Using one-step affinity purification, we have isolated enzymatically active BHC110-containing complexes. Here, we detail the methods used for the isolation and characterization of these histone demethylase complexes from a human stable cell line. (c) 2006 Elsevier Inc. All rights reserved.

引用

页码：327 / 330

页数：4

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