Determination of glyoxylyl-peptide concentration using oxime chemistry and RP-HPLC analysis

被引：7

作者：

Garcia, Jean-Michel ^{[1
]}

Far, Samia ^{[1
]}

Diesis, Eric ^{[1
]}

Melnyk, Oleg ^{[1
]}

机构：

[1] CNRS, UMR 8161, Inst Biol, F-59021 Lille, France

来源：

JOURNAL OF PEPTIDE SCIENCE | 2006年 / 12卷 / 11期

关键词：

glyoxylyl-peptide; oxime; O-benzylhydroxylamine;

D O I：

10.1002/psc.791

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Glyoxylyl-peptides are useful peptide derivatives in the context of hydrazone, oxime or thiazolidine ligations. We describe a method for the determination of glyoxylyl-peptide concentration based on the reaction of the alpha-oxo aldehyde group with an excess of O-benzylhydroxylamine. The amount of O-benzylhydroxylamine necessary to convert the alpha-oxo aldehyde group into the corresponding O-benzyloxime was determined by RP-HPLC analysis and corresponded to the quantity of glyoxylyl-peptide used in the experiment. The method is rapid, sensitive, accurate and allows the automated analysis of several samples. Copyright (c) 2006 European Peptide Society and John Wiley & Sons, Ltd.

引用

页码：734 / 738

页数：5

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