High frequency plant regeneration from the cotyledonary node of common bean

被引:23
作者
Dang, W. [1 ,2 ]
Wei, Z. M. [1 ]
机构
[1] Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai 200032, Peoples R China
[2] Chinese Acad Sci, Grad Sch, Shanghai 200032, Peoples R China
关键词
bud induction; Phaseolus vulgaris; shoot development; silver nitrate; thidiazuron; PHASEOLUS-VULGARIS L; SHOOT FORMATION; INTACT SEEDLINGS; SILVER-NITRATE; CULTURES; ORGANOGENESIS; THIDIAZURON; ACUTIFOLIUS; MERISTEMS; EXPLANTS;
D O I
10.1007/s10535-009-0056-5
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
An efficient regeneration system for Phaseolus vulgaris was developed from mature seeds germinated on Murashige and Skoog (MS) medium supplemented with thidiazuron or N-6-benzylaminopurine (BA) for 6 d. Using cotyledonary nodes, multiple buds were induced on the MS medium supplemented with 5.0 mg dm(-3) BA with the induction frequency 71.9 % after 4-week culture. The buds were then transferred onto shoot formation medium containing 1.0 mg dm(-3) BA, 0.1 mg dm(-3) gibberellic acid and 2.0 mg dm(-3) silver nitrate. The addition of AgNO3 enhanced the frequency of the shoot formation from 61.3 to 87.6 %. Root induction medium was half-strength MS medium with 0.75 mg dm(-3) indolebutyric acid and 0.02 mg dm(-3) BA. The average root frequency was 84.3 %. The regenerated plantlets with healthy roots grew successfully when transferred to soil. Using this system we obtained over 10 regenerated plantlets from one explant.
引用
收藏
页码:312 / 316
页数:5
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