Magnetic-nanoparticles-based fluorescent immunoassay for individual and simultaneous determination of dichlorvos and paraoxon in milk

被引：9
作者：
Yaneva, Marina [1 ]
Ivanov, Yavor [1 ]
Todorov, Nikola [2 ]
Godjevargova, Tzonka [1 ]
机构：
[1] Univ Prof Dr Asen Zlatarov, Dept Biotechnol, Burgas, Bulgaria
[2] Univ Prof Dr Asen Zlatarov, Dept Ecol, Burgas, Bulgaria
来源：
FOOD AND AGRICULTURAL IMMUNOLOGY | 2018年 / 29卷 / 01期
关键词：
Paraoxn; dichlorvos; milk; immunoassay; fluorescence; magnetic nanoparticles; LIQUID-MEMBRANE ASSAY; ORGANOPHOSPHORUS PESTICIDES; ELISA; BEADS;
D O I：
10.1080/09540105.2017.1368458
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
A sensitive and rapid magnetic-nanoparticles-based fluorescent immunoassay (MNPs-FIA) for individual and simultaneous determination of dichlorvos (DDVP) and paraoxon in untreated raw milk was developed. The conjugates DDVP-cBSA (cationized bovine serum albumin) and paraoxon-BSA (bovine serum albumin) were labeled by fluorescent dyes. The optimal amount of immobilized polyclonal anti-DDVP and anti-paraoxon antibodies on MNPs (0.375mg Ab-MNPs) and optimal concentrations of DDVP-cBSA-FITC (18 mu g/mL) and paraoxon-BSA-FITC (22 mu g/mL) for MNPs-FIA were determined. The calibration curves of individual and simultaneous immunoassays of DDVP and paraoxon were investigated. The IC50 value of the individual paraoxon assay in raw cow's milk was 60ng/mL and linear range 2-300ng/mL. The IC50 value of the individual DDVP assay was 70ng/mL and linear range 5-300ng/mL. The IC50 values of the paraoxon and DDVP (100 and 120ng/mL respectively) in simultaneous assay were higher than those of the individual ones, and their linear ranges were 10-400ng/mL.
引用
收藏
页码：228 / 243
页数：16
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