Study of the binding environment of α-factor in its G protein-coupled receptor using fluorescence spectroscopy

被引:20
作者
Ding, FX
Lee, BK
Hauser, M
Patri, R
Arshava, B
Becker, JM
Naider, F [1 ]
机构
[1] CUNY Coll Staten Isl, Dept Chem, Staten Isl, NY 10314 USA
[2] Univ Tennessee, Dept Microbiol, Knoxville, TN 37996 USA
来源
JOURNAL OF PEPTIDE RESEARCH | 2002年 / 60卷 / 01期
关键词
alpha-factor receptor; binding environment; fluorescence quenching; fluorescent pheromone synthesis; Saccharomyces cerevisiae;
D O I
10.1034/j.1399-3011.2002.21004.x
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Mating in Saccharomyces cerevisiae is induced by the interaction of alpha-factor (W(1)H(2)W(3)L(4)Q(5)L(6)K(7)P(8)G(9)Q(10)P(11)M(12)y(13)) with its cognate G protein-coupled receptor (Ste2p). Fifteen fluorescently labeled analogs of a-factor in which the 7-nitrobenz-2-oxa-1,3-diazol-4-yl (NBD) group was placed at the alphaN-terminus and in side-chains at positions 1, 3, 4, 6, 7, 12 and 13 were synthesized and assayed for biological activity and receptor affinity. Eleven of the analogs retained 6-60% of the biological activity of the a-factor, as judged using a growth arrest assay. The binding affinities depended on the position of NBD attachment in the peptide and the distance of the tag from the backbone. Derivatization of the positions 3 and 7 side-chains with the NBD group resulted in analogs with affinities of 17-35% compared with that of a-factor, None of the other NBD-containing agonists had sufficient receptor affinity or strong enough emission for fluorescence analysis. The position 3 and 7 analogs were investigated using fluorescence spectroscopy and collisional quenching by KI in the presence of Ste2p in yeast membranes. The results showed that the lambda(max) of NBD in the position 7 side-chain shifted markedly to the blue (510 nm) when separated by 4 or 6 bonds from the peptide backbone and that this probe was shielded from Dates: quenching by KI. In contrast, separation by 3, 5, 10 or more bonds resulted in lambda(max) (approximate to540 nm) and collisional quenching constants consistent with increasing degrees of exposure. The NBD group in the position 3 side-chain was also found to be blue shifted (lambda(max) = 520 nm) and shielded from solvent. These results indicate that the position 7 side-chain is likely interacting with a pocket formed by extracellular domains of Ste2p, whereas the side-chain of Trp(3) is in a hydrophobic pocket possibly within the transmembrane region of the receptor.
引用
收藏
页码:65 / 74
页数:10
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