Blastocyst-like embryo surrogates from stem cells in implantation models

被引:1
作者
Tian, Siyu [1 ]
Ruan, Hanzhang [1 ]
Yeung, William Shu-Biu [1 ,2 ,3 ,4 ]
Lee, Yin Lau [1 ,2 ,3 ,5 ]
机构
[1] Univ Hong Kong, Li Ka Shing Fac Med, Dept Obstet & Gynaecol, Hong Kong 999077, Peoples R China
[2] Univ Hong Kong, Shenzhen Hosp, Reprod Med Ctr, Shenzhen Key Lab Fertil Regulat, Shenzhen 518053, Peoples R China
[3] Ctr Translat Stem Cell Biol, Bldg 17W, Hong Kong Sci & Technol Pk, Hong Kong 999077, Peoples R China
[4] Univ Hong Kong Shenzhen Hosp, Shenzhen Key Lab Fertil Regulat, Shenzhen 518053, Peoples R China
[5] Univ Hong Kong, Li Ka Shing Fac Med, Dept Obstet & Gynaecol, 21 Sassoon Rd, Hong Kong 999077, Peoples R China
关键词
In vitro implantation models; Stem cells; Embryo surrogates; Endometrium; Trophoblastic spheroid; Blastoids; ENDOMETRIAL EPITHELIAL-CELLS; IN-VITRO MODEL; SPHEROID ATTACHMENT; TROPHOBLAST DIFFERENTIATION; CHORIOCARCINOMA CELL; SELF-ORGANIZATION; DOWN-REGULATION; CULTURE MODELS; ORGAN-CULTURE; GROWTH-FACTOR;
D O I
10.1097/RD9.0000000000000042
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
Successful embryo implantation requires highly coordinated maternal-embryo interactions. Implantation failure is a major factor contributing to infertility. However, the mechanism underlying implantation failure remains unclear. An improved understanding of the early implantation process not only improves the success rate of assisted reproductive treatments but also helps in studying the pathophysiology of reproductive disorders. Owing to ethical concerns, in vivo studies of human embryo implantation are not feasible. However, the results obtained from animal models cannot be directly applied to humans. Over the years, in vitro implantation models have been developed to investigate implantation mechanisms. In this review, we discuss the use of different models for generating embryo-like surrogates to study early embryo development and implantation in vitro, with a specific focus on stem cell-derived blastocyst-like embryo surrogates. There is no definitive evidence that the recently established embryo-like models re-capitulate all developmental events of human embryos during the peri-implantation stage. Regardless, stem cell-derived embryo surrogates are the most valuable tools for studying the mechanisms of early cell lineage differentiation and developmental failures during implantation.
引用
收藏
页码:225 / 233
页数:9
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