Glucose Gradients Influence Zonal Matrix Deposition in 3D Cartilage Constructs

被引:0
作者
Spitters, Tim W. G. M. [1 ]
Mota, Carlos M. D. [2 ]
Uzoechi, Samuel C. [1 ]
Slowinska, Barbara [1 ]
Martens, Dirk E. [3 ]
Moroni, Lorenzo [2 ]
Karperien, Marcel [1 ]
机构
[1] Univ Twente, Dept Dev BioEngn, MIRA Inst, NL-7500 AE Enschede, Netherlands
[2] Univ Twente, Dept Tissue Regenerat, MIRA Inst, NL-7500 AE Enschede, Netherlands
[3] Wageningen Univ, Dept Agrotechnol & Food Sci, Bioproc Engn Grp, NL-6700 AP Wageningen, Netherlands
关键词
TISSUE-ENGINEERED CARTILAGE; SEEDED ALGINATE CONSTRUCTS; ARTICULAR CHONDROCYTES; OXYGEN-CONSUMPTION; GENE-EXPRESSION; CULTURE; HYPOXIA; DIFFERENTIATION; OSTEOARTHRITIS; MITOCHONDRIA;
D O I
10.1089/ten.tea.2014.0059
中图分类号
Q813 [细胞工程];
学科分类号
摘要
Reproducing the native collagen structure and glycosaminoglycan (GAG) distribution in tissue-engineered cartilage constructs is still a challenge. Articular cartilage has a specific nutrient supply and mechanical environment due to its location and function in the body. Efforts to simulate this native environment have been reported through the use of bioreactor systems. However, few of these devices take into account the existence of gradients over cartilage as a consequence of the nutrient supply by diffusion. We hypothesized that culturing chondrocytes in an environment, in which gradients of nutrients can be mimicked, would induce zonal differentiation. Indeed, we show that glucose gradients facilitating a concentration distribution as low as physiological glucose levels enhanced a zonal chondrogenic capacity similar to the one found in native cartilage. Furthermore, we found that the glucose consumption rates of cultured chondrocytes were higher under physiological glucose concentrations and that GAG production rates were highest in 5 mM glucose. From these findings, we concluded that this condition is better suited for matrix deposition compared to 20 mM glucose standard used in a chondrocyte culture system. Reconsidering the culture conditions in cartilage tissue engineering strategies can lead to cartilaginous constructs that have better mechanical and structural properties, thus holding the potential of further enhancing integration with the host tissue.
引用
收藏
页码:3270 / 3278
页数:9
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