Chlorophyll degradation and carotenoid biosynthetic pathways: Gene expression and pigment content in broccoli during yellowing

被引：72

作者：

Luo, Feng ^{[1
]}

Cheng, Shun-Chang ^{[1
]}

Cai, Jia-Hui ^{[1
]}

Wei, Bao-Dong ^{[1
]}

Zhou, Xin ^{[1
]}

Zhou, Qian ^{[1
]}

Zhao, Ying-Bo ^{[1
]}

Ji, Shu-Juan ^{[1
]}

机构：

[1] Shenyang Agr Univ, Dept Food Sci, 120 Dongling Rd, Shenyang 110866, Liaoning, Peoples R China

来源：

FOOD CHEMISTRY | 2019年 / 297卷

基金：

国家重点研发计划;

关键词：

Broccoli; Gene expression; Temperature; Ultrastructure; Yellowing; HARVESTING COMPLEX-II; ZEAXANTHIN-EPOXIDASE; ABSCISIC-ACID; ARABIDOPSIS; REDUCTASE; LIGHT; ANTHOCYANINS; ACCUMULATION; METABOLISM; BOTRYTIS;

D O I：

10.1016/j.foodchem.2019.124964

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Broccoli undergoes yellowing in unfavorable conditions, thereby diminishing the sensory quality and commodity value. This study aimed to investigate systematically cellular and/or biomolecular changes involved in broccoli yellowing by analyzing changes in microstructural integrity, pigment content, and gene expression. On day-5 of storage at 20 degrees C, the buds turned yellow without blooming and showed structural damage; ultrastructural analysis revealed plastid transformation and abnormal chloroplast development. Genes regulating pigment content and chloroplast structure directly were identified. More specifically, BoCAO and BoNYC1 regulated chlorophyll turnover, affecting chlorophyll a and b contents. Changes in the beta-cryptoxanthin content were influenced by the combined action of up-(BoHYD) and downstream (BoZEP) genes. BoZEP and BoVDE were activated after cold-temperature induction. High BoHO1 expression delayed yellowing at low temperature, inducing BoZEP expression. Color intensity correlated significantly with the chlorophyll b, beta-cryptoxanthin, and beta-carotene contents, which were associated with increased yellowing of plant tissues.

引用

页数：9

共 39 条

[1] Impact of UV-B irradiation on chlorophyll degradation and chlorophyll-degrading enzyme activities in stored broccoli (Brassica oleracea L. Italica Group) florets [J].

Aiamla-or, Sukanya ;

Kaewsuksaeng, Samak ;

Shigyo, Masayoshi ;

Yamauchi, Naoki .

FOOD CHEMISTRY, 2010, 120 (03) :645-651

[2] Aldehyde oxidase in wild type and aba1 mutant leaves of Nicotiana plumbaginifolia [J].

Akaba, S ;

Leydecker, MT ;

Moureaux, T ;

Oritani, T ;

Koshiba, T .

PLANT AND CELL PHYSIOLOGY, 1998, 39 (12) :1281-1286

[3] Hydrogen sulfide alleviates the aluminum-induced changes in Brassica napus as revealed by physiochemical and ultrastructural study of plant [J].

Ali, Basharat ;

Qian, Ping ;

Sun, Rui ;

Farooq, Muhammad A. ;

Gill, Rafaqat A. ;

Wang, Jian ;

Azam, Muhammad ;

Zhou, Weijun .

ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH, 2015, 22 (04) :3068-3081

[4] COPPER ENZYMES IN ISOLATED CHLOROPLASTS - POLYPHENOLOXIDASE IN BETA-VULGARIS [J].

ARNON, DI .

PLANT PHYSIOLOGY, 1949, 24 (01) :1-15

[5] Biliverdin reductase:: A major physiologic cytoprotectant [J].

Barañano, DE ;

Rao, M ;

Ferris, CD ;

Snyder, SH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (25) :16093-16098

[6] The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments [J].

Bustin, Stephen A. ;

Benes, Vladimir ;

Garson, Jeremy A. ;

Hellemans, Jan ;

Huggett, Jim ;

Kubista, Mikael ;

Mueller, Reinhold ;

Nolan, Tania ;

Pfaffl, Michael W. ;

Shipley, Gregory L. ;

Vandesompele, Jo ;

Wittwer, Carl T. .

CLINICAL CHEMISTRY, 2009, 55 (04) :611-622

[7] Effect of illumination and chlorophylls on stability of tomato carotenoids [J].

Calvo, Marta M. ;

Santa-Maria, Guillermo .

FOOD CHEMISTRY, 2008, 107 (04) :1365-1370

[8] Regulation of Carotenoid Composition and Shoot Branching in Arabidopsis by a Chromatin Modifying Histone Methyltransferase, SDG8 [J].

Cazzonelli, Christopher I. ;

Cuttriss, Abby J. ;

Cossetto, Susan B. ;

Pye, William ;

Crisp, Peter ;

Whelan, Jim ;

Finnegan, E. Jean ;

Turnbull, Colin ;

Pogson, Barry J. .

PLANT CELL, 2009, 21 (01) :39-53

[9] Probing the Effect of the Non-Active-Site Mutation Y229W in New Delhi Metallo-β-lactamase-1 by Site-Directed Mutagenesis, Kinetic Studies, and Molecular Dynamics Simulations [J].

Chen, Jiao ;

Chen, Hui ;

Shi, Yun ;

Hu, Feng ;

Lao, Xingzhen ;

Gao, Xiangdong ;

Zheng, Heng ;

Yao, Wenbing .

PLOS ONE, 2013, 8 (12)

[10]

Fu X., 2017, FOOD CHEM, V239, P892

← 1 2 3 4 →